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| Status |
Public on Jan 12, 2024 |
| Title |
Crosstalk between chromatin and the transcription factor Svb |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
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| Summary |
The transcription factor Shavenbaby (Svb), the only member of the OvoL family in Drosophila, controls intestinal stem cell differentiation. Post-translational modification of Svb produces two protein isoforms, Svb-ACT and Svb-REP, which promote intestinal stem cell renewal or differentiation, respectively. Using engineered cell lines, we express either isoform to define their mode of action, and develop an unbiased method to identify Svb target genes in intestinal cells. Within a given cell type, Svb-ACT and Svb-REP antagonistically regulate the expression of a set of target genes, binding specific enhancers whose accessibility is constrained by. During intestinal differentiation, the set of target genes progressively changes, together with chromatin accessibility. Moreover, Svb-REP binding stabilizes three-dimensional enhancer-promoter loops, while influencing the local chromatin landscape to repress target genes. We propose that SvbACT-to-REP switch promotes enterocyte differentiation of intestinal stem cells through direct gene regulation and chromatin remodeling.
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| Overall design |
We used the technology of Hi-C to quantifiy long-range interactions resulting from the 3D organization of the genome, in drosophila S2 cells expressing the Svb repressor or activator isoforms (Svb-REP and Svb ACT, respectively)
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| Contributor(s) |
Alexandra M, Olivier F, David D, Olivier C, Cedric P |
| Citation(s) |
38174321 |
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| Submission date |
Dec 28, 2022 |
| Last update date |
Jan 16, 2024 |
| Contact name |
Cedric Polesello |
| Organization name |
Centre de Biologie Intégrative de Toulouse
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| Street address |
118 Route de Narbonne
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| City |
Toulouse |
| ZIP/Postal code |
310362 |
| Country |
France |
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| Platforms (1) |
| GPL32518 |
DNBSEQ-T7 (Drosophila melanogaster) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA916512 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE221863_RAW.tar |
449.5 Mb |
(http)(custom) |
TAR (of HIC) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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