|
| Status |
Public on Jun 17, 2010 |
| Title |
T7-SAGE transcriptome of cleft versus end bud epithelial cells of embryonic mouse salivary glands |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by SAGE
|
| Summary |
Mus musculus embryonic submandibular salivary glands from embryonic day 12.5 embryos were laser microdissected to isolate epithelial cells from regions adjacent to forming clefts or from the peripheral end bud region. The isolated region-specific samples were then subjected to T7-SAGE transcriptome analysis.
|
| |
|
| Overall design |
Toluidine blue-stained cryostat sections of embryonic day 12.5 submandibular salivary glands were used for laser microdissection using an LMD system (Leica) with a pulsed UV laser on an upright automated microscope. A total of approximately 500 cells from a region immediately adjacent to a forming cleft or from a peripheral end bud region were excised by the laser and transferred to caps of 0.5 ml PCR tubes. SAGE libraries were constructed from the microdissected cleft and bud epithelia cells. SAGE tags were identified using the National Center for Biotechnology Information (NCBI) tag map set for UniGene.
|
| |
|
| Contributor(s) |
Sakai T |
| Citation(s) |
20671187 |
| |
| Submission date |
Jun 15, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Takayoshi Sakai |
| E-mail(s) |
[email protected]
|
| Phone |
81-6-6879-2275
|
| Fax |
81-6-6879-2279
|
| Organization name |
Osaka Univ Grad Sch Dent
|
| Department |
Oral-Facial Disorders
|
| Street address |
1-8 Yamadaoka
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
5650871 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL11 |
SAGE:10:NlaIII:Mus musculus |
|
| Samples (2) |
| GSM555989 |
T7-SAGE transcriptome of cleft epithelial cells of embryonic mouse salivary glands |
| GSM555990 |
T7-SAGE transcriptome of end bud epithelial cells of embryonic mouse salivary glands |
|
| Relations |
| BioProject |
PRJNA127655 |
