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| Status |
Public on Dec 19, 2012 |
| Title |
S. coelicolor M145 SMM medium time course study (Culture # 1) |
| Organism |
Streptomyces coelicolor |
| Experiment type |
Expression profiling by array
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| Summary |
Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarrays to investigate the temporal transcriptome profiles of S. coelicolor A3(2) wild type and regulatory mutants.
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| Overall design |
The time-course study involved analysis of 9 samples (14hr, 15hr, 16hr, 17hr, 18hr, 19hr, 20hr, 21hr, 40hr). Samples (14hr, 15hr, 17hr, 20hr, 40hr) were analyzed on 2 arrays. Samples (18hr, 19hr, 21hr) were analyzed on 3 arrays. Genomic DNA of S. coelicolor wild type (M145) was used as a reference for all the arrays. cDNA was labeled with Alexa647 and genomic DNA was labeled with Cy3.
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| Contributor(s) |
Takano E, Hu W |
| Citation(s) |
20955611 |
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| Submission date |
Jun 16, 2010 |
| Last update date |
Mar 20, 2013 |
| Contact name |
Marlene Castro-Melchor |
| Organization name |
University of Minnesota
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| Department |
Department of Chemical Engineering and Materials Science
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| Lab |
Hu group
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| Street address |
421 Washington Ave SE
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| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
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| Platforms (1) |
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| Samples (20)
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| GSM557003 |
S_coelicolor_M145_14hr_replicate1, culture #1 |
| GSM557004 |
S_coelicolor_M145_14hr_replicate2, culture #1 |
| GSM557005 |
S_coelicolor_M145_15hr_replicate1, culture #1 |
| GSM557006 |
S_coelicolor_M145_15hr_replicate2, culture #1 |
| GSM557007 |
S_coelicolor_M145_16hr, culture #1 |
| GSM557008 |
S_coelicolor_M145_17hr_replicate1, culture #1 |
| GSM557009 |
S_coelicolor_M145_17hr_replicate2, culture #1 |
| GSM557010 |
S_coelicolor_M145_18hr_replicate1, culture #1 |
| GSM557011 |
S_coelicolor_M145_18hr_replicate2, culture #1 |
| GSM557012 |
S_coelicolor_M145_18hr_replicate3, culture #1 |
| GSM557013 |
S_coelicolor_M145_19hr_replicate1, culture #1 |
| GSM557014 |
S_coelicolor_M145_19hr_replicate2, culture #1 |
| GSM557015 |
S_coelicolor_M145_19hr_replicate3, culture #1 |
| GSM557016 |
S_coelicolor_M145_20hr_replicate1, culture #1 |
| GSM557017 |
S_coelicolor_M145_20hr_replicate2, culture #1 |
| GSM557018 |
S_coelicolor_M145_21hr_replicate1, culture #1 |
| GSM557019 |
S_coelicolor_M145_21hr_replicate2, culture #1 |
| GSM557020 |
S_coelicolor_M145_21hr_replicate3, culture #1 |
| GSM557021 |
S_coelicolor_M145_40hr_replicate1, culture #1 |
| GSM557022 |
S_coelicolor_M145_40hr_replicate2, culture #1 |
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| This SubSeries is part of SuperSeries: |
| GSE22399 |
S. coelicolor M145 SMM medium time course study |
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| Relations |
| BioProject |
PRJNA129257 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22394_RAW.tar |
88.8 Mb |
(http)(custom) |
TAR (of GPR, TXT) |
| Processed data included within Sample table |
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