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| Status |
Public on Oct 03, 2024 |
| Title |
Microarray analysis of human cord blood derived mast cells (hCBMC) activated by Interleukin-33 (IL-33) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Mast cells respond to acute and chronic exposure to IL-33 by differing the expression of their receptors, cytokines and proteases.
We aim to characterize the distinct acute and chronic responses of mast cells to IL-33 by analyzing hCBMC expression profile of pro- and anti- inflammatory mediators.
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| Overall design |
CD34+ cells were isolated from human umbilical cord blood and differentiated into mast cells using rhIL-6 and rh-SCF for 6-8 weeks. hCBMCs were stimulated with 10 and 20 ng/mL of IL-33 for 6 and 24 hours each. Total RNA was extracted from hCBMCs and untreated controls and hybridized using Affymetrix Human Gene ST 1.0 arrays.
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| Contributor(s) |
Bakhashab S, Banafea GH, Ahmed F, Bagatian N, Subhi O, Gauthaman K, Rasool M, Schulten H, Pushparaj PN |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jan 30, 2023 |
| Last update date |
Oct 04, 2024 |
| Contact name |
Sherin Bakhashab |
| E-mail(s) |
[email protected]
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| Organization name |
King Abdulaziz University
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| Department |
Biochemistry
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| Street address |
Abdullah Sulyman
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| City |
Jeddah |
| ZIP/Postal code |
21589 |
| Country |
Saudi Arabia |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA929544 |
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