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| Status |
Public on Feb 05, 2005 |
| Title |
HOXA5 transcriptional targets |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
HOXA5 inducible cell line Hs578T was established and cultured as described previously (MCB 2004. 24:924-935). 2×106 cells were seeded onto a 10-cm cell culture dish at 24h before induction. Two batches of RNA from HOXA5-inducible cells (0 h, 6 h and 9 h) were purified for repeating the microarray hybridization experiments once using Affymatrix Chips.Total RNA was extracted using TRIzol reagent (Gibco BRL, Life Technologies, Grand Island, NY) and purified using the RNeasy Mini Kit (Qiagen, Valencia, CA).
Keywords: time-course
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| Contributor(s) |
Chen H, Rubin E, Zhang H, Chung S, Jie CC, Garrett E, Biswal S, Sukumar S |
| Citation(s) |
15757903 |
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| Submission date |
Feb 03, 2005 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Hexin Chen |
| E-mail(s) |
[email protected]
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| Phone |
410-614-4075
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| Organization name |
Johns Hopkins University
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| Department |
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| Street address |
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21231-1000 |
| Country |
USA |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA91507 |
| Supplementary data files not provided |
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