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| Status |
Public on Jan 03, 2012 |
| Title |
Global transcriptomic profiling of lactacystin-mediated neuronal death |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Inhibition of proteasome degradation pathway has been implicated in neuronal cell death leading to neurodegenerative diseases such as Parkinson’s disease and Alzheimer’s disease. Pharmacological proteasomal inhibitors such as lactacystin can induce apoptosis in cultured mouse cortical neurons through the activation of caspase-3. Furthermore, proteasomal inhibitors are also reported to mediate deleterious alterations in cell cycle regulation, inflammatory processes and protein aggregation and trigger the cell death pathway.
We discovered by microarray analysis that lactacystin treatment modulates the expression of both potentially neuroprotective as well as pro-apoptotic genes in neurons. However, the genes, upon transcriptional modulation, contribute to proteasomal inhibition-induced apoptosis, remains unidentified. By employing microarray analysis to decipher the time-dependent changes in transcription of these genes in cultured cortical neurons, we discovered different groups of genes were transcriptionally regulated at different phases of lactacystin-induced cell death.
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| Overall design |
Microarray analysis was carried out using 10 murine genome U74A and U74Av2 Genechips array (Affymetrix, Santa Clara, CA). The assignment of the arrays (GeneChip) was as follows: Control at 24 h (n=2), 48 h (n=2); exposure to 1 μM lactacystin for 24 h (n=3) and 48 h (n=3).
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| Contributor(s) |
Cheung NS, Chen MJ, Peng ZF, Manikandan J |
| Citation(s) |
20683911 |
| |
| Submission date |
Jun 21, 2010 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Minghui Jessica Chen |
| Organization name |
Menzies Research Institute
|
| Department |
Neuroscience group
|
| Lab |
A/P Steve Cheung
|
| Street address |
Menzies Research Institute, University of Tasmania, Private Bag 24
|
| City |
Hobart |
| State/province |
Tasmania |
| ZIP/Postal code |
7001 |
| Country |
Australia |
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| Platforms (2) |
| GPL32 |
[MG_U74A] Affymetrix Murine Genome U74A Array |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA128621 |
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