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| Status |
Public on Mar 12, 2024 |
| Title |
Disruption of RNAPII transcription elongation links Oncogenic splicing factor mutations to replciation stress and targetable alterations in chromatin landscape [Long read sequencing] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To understand how SF3B1K700E impacts co-transcriptional splicing, we peformed long read sequencing of RNA (nSRS-seq) in K562 cells (wild type and SF3B1K700E mutant) at 4 days after mutant allele expression
We then performed co-transcriptional splicing efficiency analysis using data obtained from Long read sequencing
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| |
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| Overall design |
Co-transcriptional splicing analysis using Nascent/Chromatin bound RNA by Pacbio Long read sequencing in K562 (wild type vs SF3B1K700E mutant) cells
|
| Web link |
https://www.biorxiv.org/content/10.1101/2023.02.25.530019v3.full
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| |
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| Contributor(s) |
Prajwal B, Manoj P |
| Citation(s) |
38521065 |
| |
| Submission date |
Feb 23, 2023 |
| Last update date |
Jun 11, 2024 |
| Contact name |
MANOJ PILLAI |
| E-mail(s) |
[email protected]
|
| Organization name |
YALE UNIVERSITY
|
| Lab |
PILLAI LAB
|
| Street address |
300 GEORGE STREET #786
|
| City |
NEW HAVEN |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (4)
|
| GSM7061458 |
K562 cells, SF3B1 wild type, 4 days, replicate 1 [LRSseq] |
| GSM7061459 |
K562 cells, SF3B1 wild type, 4 days, replicate 2 [LRSseq] |
| GSM7061460 |
K562 cells, SF3B1 K700E, 4 days, replicate 1 [LRSseq] |
| GSM7061461 |
K562 cells, SF3B1 K700E, 4 days, replicate 2 [LRSseq] |
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| This SubSeries is part of SuperSeries: |
| GSE226003 |
Disruption of RNAPII transcription elongation links Oncogenic splicing factor mutations to replciation stress and targetable alterations in chromatin landscape. |
|
| Relations |
| BioProject |
PRJNA939533 |
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