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| Status |
Public on Mar 24, 2023 |
| Title |
Cappable-seq data for Sulfolobus islandicus REY15A and cbp1 deletion strains |
| Organism |
Sulfolobus islandicus REY15A |
| Experiment type |
Other
|
| Summary |
Chromatin proteins competes with the transcription machinery for access to genomic DNA and suppress cryptic promoters. CRISPR arrays form the physical memory of CRISPR adaptive immune systems. The incorporation of virus-derived AT-rich DNA into CRISPR arrays renders them prone to harbouring cryptic promoters. Sulfolobales feature extremely long CRISPR arrays spanning several kilobases as well as a CRISPR-specific chromatin protein termed Cbp1. Altered Cbp1 expression affects transcription from CRISPR arrays in multiple ways, but the mechanistic basis remains to be understood. Here, we show that Cbp1 recruits the general chromatin protein Cren7 to form a heteromeric chromatin complex at CRISPR arrays. Cbp1-CreN7 chromatinisation plays a dual role in the transcription of CRISPR array. It suppresses spurious transcription from cryptic CRISPR array-internal promoters via steric occlusion of the transcription machinery while enhancing transcription from promoters in the CRISPR leaders. Our results show that Cbp1-CreN7 chromatinization drives the coordinated transcription of long CRISPR arrays. Additional binding sites of Cbp1 associated with transposases and the leaders of alternative CRISPR arrays hint on a wider regulatory function of Cbp1 linking defense systems and mobile genetic elements.
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| Overall design |
Cappable-seq data for Sulfolobus islandicus REY15A Δcbp1 strain and the parental strain E234 to map 5'-ends of small (20-200 nt) 5'-triphosphorylated RNAs
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| Contributor(s) |
Werner F, Blombach F |
| Citation(s) |
38388540 |
| |
| Submission date |
Feb 24, 2023 |
| Last update date |
Mar 07, 2024 |
| Contact name |
Finn Werner |
| E-mail(s) |
[email protected]
|
| Organization name |
University College London
|
| Department |
Institute of Structural and Molecular Biology
|
| Street address |
Gower Street
|
| City |
London |
| ZIP/Postal code |
WC1E 6BT |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL33183 |
Illumina NextSeq 500 (Sulfolobus islandicus REY15A) |
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| Samples (4)
|
| GSM7061723 |
S.islandicus.REY15A.E234.Cappable-seq.replicate1 |
| GSM7061724 |
S.islandicus.REY15A.E234.Cappable-seq.replicate2 |
| GSM7061725 |
S.islandicus.REY15A.cbp1.Cappable-seq.replicate1 |
| GSM7061726 |
S.islandicus.REY15A.cbp1.Cappable-seq.replicate2 |
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| This SubSeries is part of SuperSeries: |
| GSE226026 |
Cbp1-Cren7 chromatinization of CRISPR arrays favours transcription from CRISPR leaders over cryptic promoters. |
|
| Relations |
| BioProject |
PRJNA938386 |
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