|
| Status |
Public on Mar 10, 2023 |
| Title |
Gene expression of Acanthamoeba castellanii under long-term co-culture condition |
| Organism |
Acanthamoeba castellanii |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The gene expression of A. castellanii cultured with low-nutrient medium and heat-killed bacteria for 21 days. The RNA-seq analysis was performed by Illumina HiSeq platform. The treated A. castellanii was compared with amoeba cultured in growth medium.
|
| |
|
| Overall design |
The A. castellanii as cultured with 20% PYG medium was diluted 1:4 by Page’s Amoeba Saline (PAS) and heat-killed E. coli. To produce the heat-killed E. coli DH5α, the bacteria with OD = 1.0 was collected by centrifugation and suspended in the corresponding medium (100% or 20% PYG medium). Then, the suspended E. coli DH5α were heated at 100℃ for 10 minutes. The heat-killed bacteria were finally diluted into 2.0×106 CFU/mL. Hundred units/ml of ampicillin were added to each medium.
|
| |
|
| Contributor(s) |
Lin W, Tsai C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 10, 2023 |
| Last update date |
Mar 13, 2023 |
| Contact name |
Wei-Chen Lin |
| E-mail(s) |
[email protected]
|
| Organization name |
National Cheng Kung University
|
| Street address |
No.1, University Road
|
| City |
Tainan City |
| ZIP/Postal code |
701 |
| Country |
Taiwan |
| |
|
| Platforms (1) |
| GPL33242 |
Illumina HiSeq 4000 (Acanthamoeba castellanii) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA943246 |
