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| Status |
Public on Oct 27, 2023 |
| Title |
Unbiased transcription factor CRISPR screen identifies ZNF800 as master repressor of enteroendocrine differentiation [scRNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Enteroendocrine cells (EECs) are hormone-producing cells residing in the epithelium of stomach, small intestine (SI) and colon. EECs regulate various aspects of metabolic activity including insulin levels, satiety, gastrointestinal secretion and motility. The generation of different EEC lineages of the SI is incompletely understood. We now report a TFome-wide CRISPR knockout screen in adult human intestinal organoids to identify dominant transcription factors controlling EEC differentiation. ZNF800 is discovered as a master repressor for endocrine lineage commitment, which particularly restricts enterochromaffin cell differentiation by directly controlling an endocrine TF network centered on PAX4. Thus, organoid models allow unbiased functional SCRISPR screens for genes that program cell differentiation.
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| Overall design |
Single-cell RNA-sequencing of WT and ZNF800-KO human small intestinal organoids
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| Contributor(s) |
Lin L, DeMartino J |
| Citation(s) |
37883554 |
| |
| Submission date |
Apr 13, 2023 |
| Last update date |
Oct 28, 2023 |
| Contact name |
Jeff DeMartino |
| Organization name |
Princess Maxima Center
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| Street address |
Heidelberglaan 25
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| City |
Utrecht |
| ZIP/Postal code |
3584 CS |
| Country |
Netherlands |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE229586 |
Unbiased transcription factor CRISPR screen identifies ZNF800 as master repressor of enteroendocrine differentiation. |
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| Relations |
| BioProject |
PRJNA955102 |
