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| Status |
Public on Dec 01, 2005 |
| Title |
Egr-1 induced gene expression changes in HUVECs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The target genes of the transcription factor early growth response-1 (EGR-1) were studied in human umbilical vein endothelial cells (HUVEC). Densely cultured HUVEC were infected with recombinant adenoviruses expressing EGR-1 or empty control viruses at a MOI of 100. RNA was isolated from the cells at the time points 16 h, 24 h and 48 h post infection. Control cells without infection were cultured in parallel and harvested at the 24 h time point. Total RNA was isolated using TRIzol (InVitrogen) and Alater (Ambion) reagents and cDNA synthesis, hybridization and microarray analysis using U133 GeneChips performed according to the Affymetrix manual. Gene expression changes were calculated as the ratio of EGR-1 or control virus (CV) infected cells to non-infected control cells.
Keywords: time-course
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| Contributor(s) |
Pomyje J, Bilban M, Hofer E |
| Citation(s) |
16818645 |
| |
| Submission date |
Feb 19, 2005 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Martin Bilban |
| E-mail(s) |
[email protected]
|
| Phone |
++43 (0)1 40400 6441
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| Organization name |
Medical University of Vienna
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| Department |
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| Street address |
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| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
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| Platforms (2) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
| GPL97 |
[HG-U133B] Affymetrix Human Genome U133B Array |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA91679 |
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