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| Status |
Public on Mar 02, 2011 |
| Title |
Microarray analysis of Wolbachia infected Anopheles gambiae Sua5B cells |
| Platform organisms |
Plasmodium falciparum; Anopheles gambiae |
| Sample organism |
Anopheles gambiae |
| Experiment type |
Expression profiling by array
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| Summary |
Wolbachia, an endosymbiotic bacterium, is being investigated as a vector control agent in several insect species. Along with the well known classical reproductive parasitism Wolbachia employs against its host to spread within the population, it is emerging that the bacteria can protect the host against pathogens and reduced pathogen transmission. Anopheles mosquitoes, which transmit malaria, have never been found to harbour Wolbachia in nature, and despite numerous transinfection attempts, no stable line has been developed.
However recently, two strains of Wolbachia, wAlbB from Aedes albopictus, and wRi from Drosophila simulans were cultured in Anopheles gambiae Sua5B cells. These cell lines provides an amenable system to study Wolbachia-Anopheles interaction in the absence of a stable transinfected line. It has been proposed that the compromised vector competence of Wolbachia infected insects is due to an up regulation of the basal immune state. We therefore completed a genome wide expression profile of Wolbachia infected Anopheles, assessing both wAlbB and wRi infected cells in parallel against uninfected Sua5B cells.
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| Overall design |
Two strains of Wolbachia, wRi from Drosophila simulans and wAlbB from Aedes albopictus were transfered into Anopheles gambiae Sua5B cells via the shell vial technique. After over 30 passages, these Wolbachia infected cells lines were then compared, in parallel, to the original uninfected Sua5B cells using Affymetrix microarrays.
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| Contributor(s) |
Hughes GL, Ren X, Ramirez JL, Bailey JA, Jedlicka AE, Rasgon JL |
| Citation(s) |
21379333 |
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| Submission date |
Jul 28, 2010 |
| Last update date |
Jan 24, 2013 |
| Contact name |
Grant Hughes |
| E-mail(s) |
[email protected]
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| Organization name |
Johns Hopkins
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| Department |
Bloomberg School of Public Health
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| Lab |
Rasgon
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| Street address |
615 N Wolfe St
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (1) |
| GPL1321 |
[Plasmodium_Anopheles] Affymetrix Plasmodium/Anopheles Genome Array |
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| Samples (9)
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| GSM571062 |
Sua5B cells infected with Wolbachia strain wAlbB from Aedes albopictus AS23 cells, replicate 1 |
| GSM571063 |
Sua5B cells infected with Wolbachia strain wAlbB from Aedes albopictus AS23 cells, replicate 2 |
| GSM571064 |
Sua5B cells infected with Wolbachia strain wAlbB from Aedes albopictus AS23 cells, replicate 3 |
| GSM571065 |
Sua5B cells infected with Wolbachia strain wRi from Drosophila simulans, replicate 1 |
| GSM571066 |
Sua5B cells infected with Wolbachia strain wRi from Drosophila simulans, replicate 2 |
| GSM571067 |
Sua5B cells infected with Wolbachia strain wRi from Drosophila simulans, replicate 3 |
| GSM571068 |
Control Anopheles gambiae Sua5B cells, replicate 1 |
| GSM571069 |
Control Anopheles gambiae Sua5B cells, replicate 2 |
| GSM571070 |
Control Anopheles gambiae Sua5B cells, replicate 3 |
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| Relations |
| BioProject |
PRJNA131309 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE23215_RAW.tar |
16.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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