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| Status |
Public on Apr 08, 2024 |
| Title |
MYCT1 Controls Environmental Sensing in human Hematopoietic Stem Cells [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We identified a novel HSC (hematopoietic stem cell) regulatory gene, MYCT1 (MYC target 1), that is selectively expressed in undifferentiated human HSPC and is critical for human HSC expansion and engraftment. To define the molecular function of MYCT1 we performed cell fractionation, immunofluorescence, and immunoprecipitation coupled with high sensitivity mass spectrometry of V5-tagged MYCT1 in KG1 hematopoietic cells and human endothelial cells (E4-immortalized HUVEC, E4EC (Butler et al. 2012)). We found that MYCT1 localizes in the endosomal membrane and interacts with vesicle trafficking regulators and signalling machinery essential for HSC and endothelial cell function. We performed RNAseq to asses the expression of the identified MYCT1 interactors in KG1 and E4EC cell lines.
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| Overall design |
RNAseq of KG1 (acute myeloid leukemia) and E4EC (E4-immortalized HUVEC) cell lines.
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| Contributor(s) |
Aguadé-Gorgorió J, Mikkola HK |
| Citation(s) |
38839950 |
| |
| Submission date |
May 11, 2023 |
| Last update date |
Jun 11, 2024 |
| Contact name |
Júlia Aguadé-Gorgorió |
| Organization name |
University of California Los Angeles
|
| Department |
Molecular, Cell and Developmental Biology
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| Lab |
Mikkola lab
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| Street address |
615 Charles E Young Drive South
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| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE232362 |
MYCT1 Controls Environmental Sensing in human Hematopoietic Stem Cells. |
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| Relations |
| BioProject |
PRJNA971732 |
