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| Status |
Public on Dec 31, 2013 |
| Title |
Global transcriptomic profiling of hypoxic ischemia in an in vivo neonatal mouse model: cortex |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Hypoxia-ischemia (HI) brain damage is one of the most common causes of neonatal brain injuries, amidst other conditions such as intrauterine infection and perinatal cerebral hemorrhage (Bracci et al., 2006). HI, occurring during the perinatal period, severely affects brain integrity resulting in detrimental long-term neurological morbidity in terms of motor, intellectual, educational and neuropsychological performance deficits (e.g. cerebral palsy, mental retardation, learning disability and epilepsy), and even neonatal mortality (Cowan et al., 2003; Ferriero, 2004; van Handel et al., 2007; Shalak and Perlman, 2004). Current therapeutic interventions fail to provide substantial reversal of HI brain injuries and improvement in overall cognitive function. Recent clinical studies demonstrated that post-HI hypothermia provide moderate neuroprotection but fail to show any significant reduction in neonatal morbidity and mortality (Shankaran et al., 2005). We would like to investigate the transcriptional effects of HI on neonatal brain, and if hypoxic pre-conditioning is beneficial to the reduction of brain damage.
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| Overall design |
Microarray analysis was performed on the cortex of neonatal brains using Illumina mouse Ref8 V2 genechips. The right common carotid artery was exposed through a ventral midline neck incision and permanently occluded by electrocoagulation, The wound was sutured and mouse pups were returned to their mother for 1.5–2 h. Sham control and pre-conditioned mice (n = 4) underwent the identical procedure, without carotid artery occlusion. Pups were then placed in an 8% O2/92% N2 humidified chamber at 37°C for 1 h with tissue extraction taking place 3h, 8h and 24h thereafter (n=4 respectively). Sham controls were included in this study too (n=4 respectively).
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| Contributor(s) |
Cheung NS, Chen M, Crack P, Manikandan J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 29, 2010 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Minghui Jessica Chen |
| Organization name |
Menzies Research Institute
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| Department |
Neuroscience group
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| Lab |
A/P Steve Cheung
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| Street address |
Menzies Research Institute, University of Tasmania, Private Bag 24
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| City |
Hobart |
| State/province |
Tasmania |
| ZIP/Postal code |
7001 |
| Country |
Australia |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (59)
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| This SubSeries is part of SuperSeries: |
| GSE23333 |
Global transcriptomic profiling of hypoxic ischemia in an in vivo neonatal mouse model |
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| Relations |
| BioProject |
PRJNA133301 |
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