|
| Status |
Public on Dec 12, 2023 |
| Title |
RNA-sequencing of epithelial cell subpopulations from human breast tissue [RNASeq_1_BRCA2] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Molecular profiling of mammary epithelial cell subpopulations from premenopausal BRCA2 mutation carriers and premenopausal non-carriers was performed to identify early transcriptional perturbations in preneoplastic BRCA2-mutant breast.
|
| |
|
| Overall design |
Mammary epithelial cell subpopulations (Lin- EPCAM+CD49f- ML, Lin- EPCAM+CD49f+ LP and Lin-EPCAM-CD49f+ basal cells) were isolated from preneoplastic breast tissue by FACS and subjected to bulk RNA-sequencing (n=3 premenopausal BRCA2 mutation carriers and n=3 premenopausal non-carriers).
** Submitter declares that the raw data files are not provided due to privacy concerns about the human samples***
|
| Web link |
https://www.nature.com/articles/s41556-023-01315-5
|
| |
|
| Contributor(s) |
Chen Y, Joyce R, Smyth GK, Visvader JE |
| Citation(s) |
38216737 |
| |
| Submission date |
May 24, 2023 |
| Last update date |
Mar 12, 2024 |
| Contact name |
Yunshun Chen |
| E-mail(s) |
[email protected]
|
| Organization name |
Walter and Eliza Hall Institute of Medical Research
|
| Department |
ACRF Cancer Biology and Stem Cells
|
| Lab |
Chen
|
| Street address |
1G Royal Parade
|
| City |
Parkville |
| State/province |
VIC |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (18)
|
| GSM7422332 |
Basal population from Patient 2397 [BRCA2-Basal-2397] |
| GSM7422333 |
LP population from Patient 2397 [BRCA2-LP-2397] |
| GSM7422334 |
ML population from Patient 2397 [BRCA2-ML-2397] |
| GSM7422335 |
Basal population from Patient 0050 [BRCA2-Basal-0050] |
| GSM7422336 |
LP population from Patient 0050 [BRCA2-LP-0050] |
| GSM7422337 |
ML population from Patient 0050 [BRCA2-ML-0050] |
| GSM7422338 |
Basal population from Patient 0004 [BRCA2-Basal-0004] |
| GSM7422339 |
LP population from Patient 0004 [BRCA2-LP-0004] |
| GSM7422340 |
ML population from Patient 0004 [BRCA2-ML-0004] |
| GSM7422341 |
Basal population from Patient 0025 [WT-Basal-0025] |
| GSM7422342 |
LP population from Patient 0025 [WT-LP-0025] |
| GSM7422343 |
ML population from Patient 0025 [WT-ML-0025] |
| GSM7422344 |
Basal population from Patient 0174 [WT-Basal-0174] |
| GSM7422345 |
LP population from Patient 0174 [WT-LP-0174] |
| GSM7422346 |
ML population from Patient 0174 [WT-ML-0174] |
| GSM7422347 |
Basal population from Patient 0399 [WT-Basal-0399] |
| GSM7422348 |
LP population from Patient 0399 [WT-LP-0399] |
| GSM7422349 |
ML population from Patient 0399 [WT-ML-0399] |
|
| This SubSeries is part of SuperSeries: |
| GSE233505 |
Identification of aberrant luminal progenitors and mTORC1 as a potential chemoprevention target in BRCA2 mutation carriers |
|
| Relations |
| BioProject |
PRJNA975846 |
Less...
More...