|
| Status |
Public on Dec 12, 2023 |
| Title |
RNA-sequencing of ERBB3hi and ERBB3lo breast luminal progenitor cells from n=3 premenopausal BRCA2 mutation carriers and n=5 non-carriers [RNASeq_3_ERBB3] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA-sequencing was performed on patient luminal progenitor breast cell subsets from premenopausal, preneoplastic BRCA2 mutation carriers and non-carriers to interrogate early transcriptional alterations in BRCA2mut/+ cells.
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| |
|
| Overall design |
Lin-EPCAM+CD49f+ERBB3hi and/or Lin-EPCAM+CD49f+ERBB3lo luminal progenitor cells were isolated from n=3 premenopausal BRCA2 mutation carriers and n=5 non-carriers by FACS, then subjected to RNA-sequencing. Fluorescence minus one (FMO) controls were used to determine ERBB3lo versus ERBB3hi cells for each patient.
** Submitter declares that the raw data files are not provided due to privacy concerns about the human samples***
|
| Web link |
https://www.nature.com/articles/s41556-023-01315-5
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| |
|
| Contributor(s) |
Chen Y, Joyce R, Pascual R, Smyth GK, Visvader JE |
| Citation(s) |
38216737 |
| |
| Submission date |
May 24, 2023 |
| Last update date |
Mar 12, 2024 |
| Contact name |
Yunshun Chen |
| E-mail(s) |
[email protected]
|
| Organization name |
Walter and Eliza Hall Institute of Medical Research
|
| Department |
ACRF Cancer Biology and Stem Cells
|
| Lab |
Chen
|
| Street address |
1G Royal Parade
|
| City |
Parkville |
| State/province |
VIC |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (14)
|
| GSM7422374 |
ERBB3-hi luminal progenitor cells from Patient 0151 [WT-hi-0151] |
| GSM7422375 |
ERBB3-hi luminal progenitor cells from Patient 0148 [WT-hi-0148] |
| GSM7422376 |
ERBB3-lo luminal progenitor cells from Patient 0111 [BRCA2-lo-0111] |
| GSM7422377 |
ERBB3-hi luminal progenitor cells from Patient 0111 [BRCA2-hi-0111] |
| GSM7422378 |
ERBB3-lo luminal progenitor cells from Patient 0046 [BRCA2-lo-0046] |
| GSM7422379 |
ERBB3-hi luminal progenitor cells from Patient 0046 [BRCA2-hi-0046] |
| GSM7422380 |
ERBB3-lo luminal progenitor cells from Patient 15.002.1 [BRCA2-lo-15.002.1] |
| GSM7422381 |
ERBB3-hi luminal progenitor cells from Patient 15.002.1 [BRCA2-hi-15.002.1] |
| GSM7422382 |
ERBB3-lo luminal progenitor cells from Patient 0034 [WT-lo-0034] |
| GSM7422383 |
ERBB3-hi luminal progenitor cells from Patient 0034 [WT-hi-0034] |
| GSM7422384 |
ERBB3-lo luminal progenitor cells from Patient 0907 [WT-lo-0907] |
| GSM7422385 |
ERBB3-hi luminal progenitor cells from Patient 0907 [WT-hi-0907] |
| GSM7422386 |
ERBB3-lo luminal progenitor cells from Patient 0010 [WT-lo-0010] |
| GSM7422387 |
ERBB3-hi luminal progenitor cells from Patient 0010 [WT-hi-0010] |
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| This SubSeries is part of SuperSeries: |
| GSE233505 |
Identification of aberrant luminal progenitors and mTORC1 as a potential chemoprevention target in BRCA2 mutation carriers |
|
| Relations |
| BioProject |
PRJNA975849 |
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