|
| Status |
Public on Dec 12, 2023 |
| Title |
RNA-sequencing of ERBB3hi and ERBB3lo breast luminal progenitor cells from n=3 premenopausal BRCA2 mutation carriers and n=3 non-carriers [RNASeq_4_ERBB3] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA-sequencing was performed on patient luminal progenitor breast cell subsets from premenopausal, preneoplastic BRCA2 mutation carriers and non-carriers to interrogate early transcriptional alterations in BRCA2mut/+ cells.
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| |
|
| Overall design |
Lin-EPCAM+CD49f+ERBB3hi and Lin-EPCAM+CD49f+ERBB3lo luminal progenitor cells were isolated from n=3 premenopausal BRCA2 mutation carriers and n=3 non-carriers by FACS, then subjected to RNA-sequencing. Fluorescence minus one (FMO) controls were used to determine ERBB3lo versus ERBB3hi cells for each patient.
** Submitter declares that the raw data files are not provided due to privacy concerns about the human samples***
|
| Web link |
https://www.nature.com/articles/s41556-023-01315-5
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| |
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| Contributor(s) |
Chen Y, Milevskiy MJ, Joyce R, Smyth GK, Visvader JE |
| Citation(s) |
38216737 |
| |
| Submission date |
May 24, 2023 |
| Last update date |
Mar 12, 2024 |
| Contact name |
Yunshun Chen |
| E-mail(s) |
[email protected]
|
| Organization name |
Walter and Eliza Hall Institute of Medical Research
|
| Department |
ACRF Cancer Biology and Stem Cells
|
| Lab |
Chen
|
| Street address |
1G Royal Parade
|
| City |
Parkville |
| State/province |
VIC |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (12)
|
| GSM7422388 |
ERBB3-lo luminal progenitor cells from Patient 0046 [BRCA2-ERBB3-lo-0046] |
| GSM7422389 |
ERBB3-hi luminal progenitor cells from Patient 0046 [BRCA2-ERBB3-hi-0046] |
| GSM7422390 |
ERBB3-lo luminal progenitor cells from Patient 01.0021.0323 [BRCA2-lo-01.0021.0323] |
| GSM7422391 |
ERBB3-hi luminal progenitor cells from Patient 01.0021.0323 [BRCA2-hi-01.0021.0323] |
| GSM7422392 |
ERBB3-lo luminal progenitor cells from Patient 0059 [BRCA2-lo-0059] |
| GSM7422393 |
ERBB3-hi luminal progenitor cells from Patient 0059 [BRCA2-hi-0059] |
| GSM7422394 |
ERBB3-lo luminal progenitor cells from Patient 0111 [WT-lo-0111] |
| GSM7422395 |
ERBB3-hi luminal progenitor cells from Patient 0111 [WT-hi-0111] |
| GSM7422396 |
ERBB3-lo luminal progenitor cells from Patient 0017 [WT-lo-0017] |
| GSM7422397 |
ERBB3-hi luminal progenitor cells from Patient 0017 [WT-hi-0017] |
| GSM7422398 |
ERBB3-lo luminal progenitor cells from Patient 0408 [WT-lo-0408] |
| GSM7422399 |
ERBB3-hi luminal progenitor cells from Patient 0408 [WT-hi-0408] |
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| This SubSeries is part of SuperSeries: |
| GSE233505 |
Identification of aberrant luminal progenitors and mTORC1 as a potential chemoprevention target in BRCA2 mutation carriers |
|
| Relations |
| BioProject |
PRJNA975848 |
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