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| Status |
Public on Dec 31, 2010 |
| Title |
Effect of JMJD2B depletion on the ER signaling pathway |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
JMJD2B is expressed in a high proportion of human breast tumors, and the expression levels significantly correlate with estrogen receptor (ER) positivity. To assess the effect of JMJD2B depletion on the ER signaling pathway, we performed genome-wide gene expression analysis using the Affymetrix Human Gene 1.0 ST array.
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| Overall design |
RNA was extracted from steroid-depleted control MCF-7 cells (control E2(-)), control MCF-7 cells treated with E2 (control E2(+)), steroid-depleted JMJD2B-depleted MCF-7 cells (siJ2B E2(-)), and JMJD2B-depleted MCF-7 cells treated with E2 (siJ2B E2(+)).
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| Contributor(s) |
Kawazu M, Saso K, Tong KI, MxQuire T, Goto K, Son D, Wakeham A, Miyagashi M, Mak TW, Okada H |
| Citation(s) |
21445275 |
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| Submission date |
Aug 05, 2010 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Hitoshi OKADA |
| Organization name |
Ontario Cancer Institute
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| Department |
Campbell Family Institute for Breast Cancer Research
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| Street address |
620 University Ave Suite 7-706
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| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G2C1 |
| Country |
Canada |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| GSM575323 |
steroid-depleted control MCF-7 cells, rep1 |
| GSM575324 |
steroid-depleted control MCF-7 cells, rep2 |
| GSM575325 |
steroid-depleted control MCF-7 cells, rep3 |
| GSM575326 |
control MCF-7 cells treated with E2, rep1 |
| GSM575327 |
control MCF-7 cells treated with E2, rep2 |
| GSM575328 |
control MCF-7 cells treated with E2, rep3 |
| GSM575329 |
steroid-depleted JMJD2B-depleted MCF-7 cells, rep1 |
| GSM575330 |
steroid-depleted JMJD2B-depleted MCF-7 cells, rep2 |
| GSM575331 |
steroid-depleted JMJD2B-depleted MCF-7 cells, rep3 |
| GSM575332 |
JMJD2B-depleted MCF-7 cells treated with E2, rep1 |
| GSM575333 |
JMJD2B-depleted MCF-7 cells treated with E2, rep2 |
| GSM575334 |
JMJD2B-depleted MCF-7 cells treated with E2, rep3 |
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| Relations |
| BioProject |
PRJNA130997 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE23445_RAW.tar |
51.3 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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