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| Status |
Public on Apr 16, 2024 |
| Title |
IL-7-primed bystander CD8 tumor-infiltrating lymphocytes optimize the antitumor efficacy of T-cell engager immunotherapy in solid tumors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Bispecific T-cell engagers (TCEs) show promising clinical efficacy in blood tumors, but their application to solid tumors with immunosuppressive environments remains challenging. Here, we show that Fc-fused IL-7 (rhIL-7-hyFc) changes the intratumoral CD8 T cell landscape, allowing TCE-immunotherapy to function effectively in solid tumors. In order to explore the transcriptomic changes induced by rhIL-7-hyFc or a combination of rhIL-7-hyFc and TCE treatment at the clonal level, we performed scRNA/TCR-seq.
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| Overall design |
Tumor-infiltrating CD8 T cells from mice bearing MC38 tumors were isolated following two treatment conditions: (1) treatment with either buffer or rhIL-7-hyFc, and (2) treatment with either rhIL-7-hyFc or a combination of rhIL-7-hyFc and TCE. For the enrichment of CD8 T cells, cells were isolated by positive selection methods with IMag (condition 1) or sorted by FACS (condition 2) after single-cell preparation. The purified CD8 T cells were analyzed by scRNA-seq paired with scTCR-seq.
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| Contributor(s) |
Lee K, Choi D, Tae N, Song HW, Park S, Kang Y, Moon D, Oh Y, Kim J, Hing D, Sohn J, Lee M, Im S, Park Y, Choi SS, Kim DH, Lee S |
| Citation(s) |
38744277 |
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| Submission date |
Jul 13, 2023 |
| Last update date |
Jun 26, 2024 |
| Contact name |
Hawon Song |
| Organization name |
Kangwon National University
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| Street address |
Gangwon Daehak-gil 1
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| City |
Chuncheon |
| ZIP/Postal code |
24341 |
| Country |
South Korea |
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| Platforms (1) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA994499 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE237266_RAW.tar |
316.1 Mb |
(http)(custom) |
TAR (of CSV, MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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