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| Status |
Public on Aug 07, 2023 |
| Title |
single nucleus RNA-seq of early development in P. miniata |
| Organism |
Patiria miniata |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To explore the evolution of different cell types across species, we compared transcriptomes between different cell types between two echinoderm species, P. miniata and S. purpuratus. snRNA-seq was used to analyze to characterize diffente cell states. For each species, the time points were integrated into a common atlas and annotated. The fully processed and annotated P. miniata and S. purpuratus atlases are available as supplementary files.
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| Overall design |
Fresh nuclei were isolated from embryonic cultures and analyzed using snRNA-seq. Two datasets were produced- an exploratory S. purpuratus dataset that was used to validate snRNA-seq methods, and a P. miniata dataset
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| Contributor(s) |
Meyer A, Ku C, Telmer C, Hatleberg W, Hinman V |
| Citation(s) |
37470227 |
| BioProject |
PRJNA826814 |
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| Submission date |
Aug 04, 2023 |
| Last update date |
Nov 06, 2023 |
| Contact name |
Hinman Veronica |
| E-mail(s) |
[email protected]
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| Organization name |
Carnegie Mellon University
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| Department |
Biological Sciences
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| Lab |
Hinman Lab
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| Street address |
587 S Negley Ave, Apt 17
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| City |
PITTSBURGH |
| State/province |
PA |
| ZIP/Postal code |
15232 |
| Country |
USA |
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| Platforms (1) |
| GPL33652 |
Illumina NovaSeq 6000 (Patiria miniata) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE240146 |
single nucleus RNA-seq of early development in P. miniata and S. purpuratus |
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