|
| Status |
Public on Mar 17, 2024 |
| Title |
Regulation by the RNA-binding protein Unkempt at its effector interface [Tail seq] |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
How RNA-binding proteins (RBPs) convey regulatory instructions to the core effectors of RNA processing is unclear. Here we document the existence and functions of a multivalent RBP–effector interface. We show that the effector interface of a conserved RBP with an essential role in metazoan development, Unkempt, is mediated by a novel type of ‘dual-purpose’ peptide motifs that can contact two different surfaces of interacting proteins. Unexpectedly, we find that the multivalent contacts do not merely serve effector recruitment but are required for the accuracy of RNA recognition by Unkempt. Systems analyses reveal that multivalent RBP–effector contacts can repurpose the principal activity of an effector for a different function, as we demonstrate for reuse of the central eukaryotic mRNA decay factor CCR4-NOT in translational control. Our study establishes the molecular assembly and functional principles of an RBP–effector interface.
|
| |
|
| Overall design |
We performed poly(A)tail-seq experiments to assess changes in poly(A) tail length of mRNAs upon induction of UNKWT, UNKdPAM2, or UNK3M for 24 hours in inducible HeLa cells. We processed two biological replicates of either Dox-induced or uninduced cells
|
| |
|
| Contributor(s) |
Shah K, He S, Turner D, Rebbani K, Corbo J, Bateman JM, Cheloufi S, Igreja C, Valkov E, Murn J |
| Citation(s) |
38605040 |
| |
| Submission date |
Aug 10, 2023 |
| Last update date |
May 01, 2024 |
| Contact name |
Shiyang He |
| E-mail(s) |
[email protected]
|
| Organization name |
UCR
|
| Department |
Biochemistry
|
| Lab |
Murn lab
|
| Street address |
3401 Watkins Drive, Boyce Hall
|
| City |
Riverside |
| State/province |
CA |
| ZIP/Postal code |
92521 |
| Country |
USA |
| |
|
| Platforms (2) |
|
| Samples (8)
|
|
| This SubSeries is part of SuperSeries: |
| GSE240571 |
Regulation by the RNA-binding protein Unkempt at its effector interface |
|
| Relations |
| BioProject |
PRJNA1004252 |
Less...
More...