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Series GSE24100 Query DataSets for GSE24100
Status Public on Oct 31, 2011
Title Isolation and characterization of cancer-associated fibroblast-like cells in the glioblastoma microenvironment
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Glioblastoma (GB) is a highly infiltrative tumor recurring in 90% of cases within a few centimeters of the resection cavity, even in cases of complete tumor resection and adjuvant chemo/radiotherapy. This observation highlights the importance of understanding this special zone of brain tissue surrounding the tumor. It is becoming clear that the non neoplastic stromal compartment of most solid cancers plays an active role in tumor proliferation, invasion and metastasis. Very little information, other than that concerning angiogenesis and immune cells, has been collected for stromal cells from GB. As part of a translational research program, we have isolated a new stromal cell population surrounding GB by computer-guided stereotaxic biopsies and primary culture. We named these cells GB-associated stromal cells (GASCs). GASCs are diploid, do not display the genomic alterations typical of GB cells and have phenotypic and functional properties in common with the cancer-associated fibroblasts (CAFs) in the stroma of carcinomas. In particular, GASCs express markers associated with CAFs such as alpha-smooth muscle actin (α-SMA) and platelet derived growth factor receptor-beta (PDGFRβ). Furthermore, as described for CAFs, GASCs have a molecular expression profile different from that of control stromal cells derived from non-GB peripheral brain tissues. GASCs were also found to have tumor-promoting effects on glioma cells in vitro and in vivo. The isolation of GASCs in a tumor of neuroepithelial origin was unexpected and further studies are required to determine their potential as a target for anti-glioma treatment.
 
Overall design 3 control stromal cells and 4 GB-associated stromal cells were analysed.
 
Contributor(s) Clavreul A, Menei P
Citation(s) 21928115
Submission date Sep 13, 2010
Last update date Feb 22, 2018
Contact name Amandine Etcheverry
Organization name CNRS UMR 6290 - IGDR
Street address 2 rue Henri Le Guilloux
City Rennes
ZIP/Postal code 35033
Country France
 
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (7)
GSM593174 control stroma cells 1
GSM593175 control stroma cells 2
GSM593176 control stroma cells 3
Relations
BioProject PRJNA130303

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE24100_RAW.tar 61.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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