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| Status |
Public on Oct 01, 2023 |
| Title |
Transcriptome analysis of Bacillus cereus strain INRA C3 in response to iron depletion and lactoferrin, accomplished by RNA sequencing |
| Organism |
Bacillus cereus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The iron-binding glycoprotein lactoferrin exhibits anti-bacterial activity. To investigate the overall response of Bacillus cereus to lactoferrin, total transcriptome analyses were performed. Growth conditions were mMOD minimal medium, minimal medium plus 10 mg/ml lactoferrin, as well as minimal medium without iron source for comparison. Differential regulation of genes involved in iron acquisition as well as genes encoding enterotoxins and further virulence factors was of special interest.
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| Overall design |
For subsequent RNA sequencing, strain INRA C3 was grown for two hours in mMOD minimal medium, mMOD-Fe (without iron source) and mMOD supplemetned with 10 mg/ml lactoferrin. Two biological replicates were prepared per growth condition.
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| Contributor(s) |
Jugert C, Didier A, Jessberger N |
| Citation(s) |
38029127 |
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| Submission date |
Aug 21, 2023 |
| Last update date |
Dec 06, 2023 |
| Contact name |
Nadja Jessberger |
| E-mail(s) |
[email protected]
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| Organization name |
University of Veterinary Medicine Hannover, Foundation
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| Department |
Institute for Food Quality and Food Safety
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| Street address |
Bischofsholer Damm 15
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| City |
Hannover |
| ZIP/Postal code |
30173 |
| Country |
Germany |
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| Platforms (1) |
| GPL26265 |
Illumina HiSeq 2500 (Bacillus cereus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1007588 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE241250_MOD_FE_vs_LF10.DE.tsv.gz |
155.6 Kb |
(ftp)(http) |
TSV |
| GSE241250_MOD_Fe_vs_LF10_22.08.22.xlsx |
310.4 Kb |
(ftp)(http) |
XLSX |
| GSE241250_MOD_vs_LF10.DE.tsv.gz |
155.1 Kb |
(ftp)(http) |
TSV |
| GSE241250_MOD_vs_LF10_22.08.22.xlsx |
309.3 Kb |
(ftp)(http) |
XLSX |
| GSE241250_MOD_vs_MOD_FE.DE.tsv.gz |
135.8 Kb |
(ftp)(http) |
TSV |
| GSE241250_MOD_vs_MOD_Fe_22.08.22.xlsx |
279.5 Kb |
(ftp)(http) |
XLSX |
| GSE241250_NG-27598.profiling-annotation.gff.gz |
323.1 Kb |
(ftp)(http) |
GFF |
| GSE241250_NG-27598.profiling-normalized.gct.gz |
249.7 Kb |
(ftp)(http) |
GCT |
| GSE241250_NG-27598.profiling-raw.gct.gz |
113.2 Kb |
(ftp)(http) |
GCT |
| GSE241250_NG-27598.profiling-stats.txt.gz |
279 b |
(ftp)(http) |
TXT |
| GSE241250_NG-27598.profiling-table.txt.gz |
278.3 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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