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| Status |
Public on Dec 06, 2023 |
| Title |
Analysis of genes differentially expressed between rete testis cells and Sertoli cells of the mouse testis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The rete testis (RT) is a region of the mammalian testis which plays an important role in testicular physiology. The RT epithelium consists of cells sharing some well-known gene markers with supporting Sertoli cells (SCs). However, little is known about the differences in gene expression between these two cell populations. Here, we used fluorescence-activated cell sorting (FACS) to obtain pure cultures of neonatal RT cells and SCs and identified differentially expressed genes (DEGs) between these cell types using RNA sequencing (RNA-seq).
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| Overall design |
Comparison of mRNA profiles of rete testis cells and Sertoli cells obtained from testes of 5-6 dpp mice using FACS and cultured for 3 days. For each cell type, three samples obtained from independently established cell cultures were used in the analysis.
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| Contributor(s) |
Malolina EA, Galiakberova AA, Mun VV, Sabirov MS, Dashinimaev EB, Kulibin AY |
| Citation(s) |
38017073 |
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| Submission date |
Sep 06, 2023 |
| Last update date |
Dec 06, 2023 |
| Contact name |
Valeru Mun |
| E-mail(s) |
[email protected]
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| Organization name |
IDB RAS
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| Street address |
st. Vavilova, 26
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| City |
Moscow |
| ZIP/Postal code |
119334 |
| Country |
Russia |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1013460 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE242442_gene_count_matrix.csv.gz |
444.2 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
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