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| Status |
Public on Jan 01, 2024 |
| Title |
CCl4 treatment led to differential lncRNA expression in murine hepatocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Five-week old male C57BL/6J mice (20 ± 2 g) were intraperitoneally injected with of 20% CCl4 solution in sterile mineral oil at a dose of 2.5 ml CCl4 per kilogram body weight twice per week for eight weeks. Afterwards, the primary hepatocytes were isolated by pronase/collagenase perfusion digestion followed by subsequent density gradient centrifugation. The isolated primary hepatocytes were counted with a hemocytometer to determine the number and percentage of viable cells using the trypan blue method. The isolated cells with the viability above 90% were used for the experiments.
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| Overall design |
Total RNA was extracted from the isolated hepatocytes with TRIzol reagent.
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| Contributor(s) |
Kang Z, Zhang J, Zang Y |
| Citation(s) |
38762176 |
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| Submission date |
Sep 20, 2023 |
| Last update date |
Jun 28, 2024 |
| Contact name |
Yuhui Zang |
| E-mail(s) |
[email protected]
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| Organization name |
Nanjing University
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| Street address |
163 Xianlin Road
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| City |
Nanjing |
| ZIP/Postal code |
210093 |
| Country |
China |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1019322 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE243644_mmhcRNA-control--mmhcRNA-model.all.lncRNA.txt.gz |
672.6 Kb |
(ftp)(http) |
TXT |
| GSE243644_mmhcRNA-control--mmhcRNA-model.all.mRNA.txt.gz |
597.1 Kb |
(ftp)(http) |
TXT |
| GSE243644_mmhcRNA-control--mmhcRNA-model.all.new_lncRNA.txt.gz |
45.6 Kb |
(ftp)(http) |
TXT |
| GSE243644_new_lncRNA_exp.txt.gz |
37.8 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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