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| Status |
Public on Oct 31, 2023 |
| Title |
Single cell ATAC-sequencing analysis of human SUV39H1-knockout CAR T compared to control CAR T cells (mock) in a xenograft model of lung adenocarcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We have observed that the inactivation of SUV39H1 enhances 41BBz-CAR T cell long-term persistence, providing protection against tumor relapses and rechallenges in a xenograft mouse model of lung adenocarcinoma. The purpuse of this study was to profile chromatin accessibility of SUV39H1-deficient compared to mock 41BBz-CAR T cells by single-cell assay for transposase accessible chromatin (scATAC-seq) on FACS-sorted T cells eight days after CAR T cell infusion into the mice.
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| Overall design |
NSG mice that received intravenous injections of tumor cells (A549) ectopically expressing CD19 that develop tumors in the lungs, were treated 21 days later with 0,9 million of 41BBz-CAR T cells (i.v.) that were SUV39H1-knockout (CRISPR/Cas9 technology) or controls CAR T cells (same CRISPR/Cas9 protocol with a sgRNA that does not have any complimentary sequence in the genome). CAR T cells were isolated from lungs at day 8 after infusion, T-cells were FACS sorted and subjected to Chromium Single Cell ATAC protocol (for each condition there were three biological replicates from pooled mice).
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| Contributor(s) |
López-Cobo S, Fuentealba JR, Gueguen P, Bonté P, Saitakis M, Amigorena S |
| Citation(s) |
37934001 |
| |
| Submission date |
Sep 26, 2023 |
| Last update date |
Jan 12, 2024 |
| Contact name |
Sebastian Amigorena |
| E-mail(s) |
[email protected]
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| Organization name |
Institut Curie
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| Lab |
INSERM - U932
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| Street address |
26 rue d'Ulm
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| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE244032 |
SUV39H1 Ablation Enhances Long-Term CAR-T Function in Solid Tumors. |
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| Relations |
| BioProject |
PRJNA1021140 |
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