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| Status |
Public on Jan 18, 2024 |
| Title |
Gene Expression profile at the single cell level of quiescent and activated muscle stem cells (MuSC) from Extraocular muscle (EOM) and Tibialis Anterior (TA) muscle |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Skeletal muscle stem cells (MuSCs) are recognised as functionally heterogeneous. Cranial MuSCs are reported to have greater proliferative and regenerative capacity when compared with those in the limb. A comprehensive understanding of the mechanisms
underlying this functional heterogeneity is lacking. Here, we have used clonal analysis, live imaging and scRNA-seq to identify crucial features that distinguish extraocular muscle (EOM) from limb muscle stem cell populations. A MyogenintdTom reporter showed that the increased proliferation capacity of EOM MuSCs correlates with deferred differentiation and lower expression of the myogenic commitment gene Myod. Unexpectedly, EOM MuSCs activated in vitro expressed a large array of extracellular matrix components
typical of mesenchymal non-muscle cells. Computational analysis underscored a distinct co-regulatory module, which is absent in limb MuSCs, as the driver of these features. The EOM transcription factor network, with Foxc1 as key player, appears to be hardwired to EOM identity as it persists during growth, disease and in vitro after several passages. Our findings shed light on how high-performing MuSCs regulate myogenic commitment by remodelling their local environment and adopting properties not generally associated with myogenic cells.
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| Overall design |
MuSCs from Tg:Pax7-nGFP mice were isolated by Fluorescence-based active cell sorting (FACS) based on GFP fluorescence. Quiescent MuSCs were immediatly processed for single-cell RNA sequencing and activated MuSCs were put in culture for 4 days before sequencing.
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| Web link |
https://journals.biologists.com/dev/article/151/4/dev202144/343118/Extraocular-muscle-stem-cells-exhibit-distinct
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| Contributor(s) |
Di Girolamo D, Benavente-Diaz M, Grimaldi A, Thomas Lopes P, Murolo M, Evano B, Gioftsidi S, Laville V, Mella S, Kuriki M, Tajbakhsh S, Comai GE |
| Citation(s) |
38240380 |
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| Submission date |
Oct 10, 2023 |
| Last update date |
Apr 18, 2024 |
| Contact name |
Glenda Evangelina Comai |
| E-mail(s) |
[email protected]
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| Organization name |
Institut Pasteur
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| Street address |
25-28 rue du Dr Roux
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| City |
Paris |
| ZIP/Postal code |
75015 |
| Country |
France |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA1026531 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE244964_RAW.tar |
227.1 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
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