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| Status |
Public on May 01, 2005 |
| Title |
Expression profiling of rpb1-11 |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Expression profiling by array
|
| Summary |
Two independent RNA preparations were used to hybridise to two microarrays one on
which wildtype was labelled with Cy5 and rpb-11 with Cy3 and the other on which dyes
were swapped.
The data were normalized using 'Lowess' (per chip per spot normalizations and checked
against luciferase spiking controls).
Using a 1.5 fold cutoff value for changes in expression levels 33 genes showed
reproducible up or down regulation, none of which are known to be involved in RNAidependent
transcriptional gene silencing.
The methodology and arrays were as previously described by Xue et al (2004).
Keywords: parallel sample
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| Contributor(s) |
Schramke V, Sheedy D, Denli AM, Bonilla C, Ekwall K, Hannon GJ, Allshire RC |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 01, 2005 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Karl Ekwall |
| E-mail(s) |
[email protected]
|
| Phone |
+46 8 6089133
|
| Organization name |
Karolinska Inst
|
| Street address |
Alfred Nobels Alle 7
|
| City |
Stockholm |
| ZIP/Postal code |
S-141 89 |
| Country |
Sweden |
| |
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| Platforms (1) |
| GPL1943 |
Eurogentec AG S. pombe ORF array |
|
| Samples (2) |
| GSM47007 |
rpb1-11 vs wt expression profiling |
| GSM47008 |
expression profiling rpb1-11 vs wt |
|
| Relations |
| BioProject |
PRJNA91187 |
