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| Status |
Public on Apr 24, 2024 |
| Title |
MiR-146a alleviates inflammatory bowel disease in mice through systematical regulation of multiple genetic networks |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Inflammatory bowel disease (IBD) is a multiple-genes-involved chronic disease and current available targeted drugs for IBD only deliver moderate efficacy. Whether there is a single gene that systematically regulates IBD is not yet known. Here we showed that the expression of miR-146a in colon was elevated in Dextran Sulfate Sodium Salt (DSS)-induced IBD mice and patients with IBD. DSS induced dramatic body weight loss and much more rectal bleeding, shorter colon length and colitis in miR-146a knock-out mice than wild type (WT) mice. The miR-146a mimics alleviated DSS-induced symptoms in both DSS-induced miR-146a-/- and WT mice. Further RNA sequencing illustrated that deficiency of miR-146a de-repressed majority of DSS-induced IBD-related genes which cover multiple genetic regulatory networks in IBD, and supplement of miR-146a mimics inhibited expression of many IBD-related genes.
DOI 10.3389/fimmu.2024.1366319
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| Overall design |
Experiment1: Bulk RNA of colon profiling data from 3 untreated wild type,3 DSS-treated wild type and 3 DSS-treated miR-146a knock-out mice. Experiment2: Bulk RNA of colon profiling data from 6 (3 females+3 males) DSS+saline treated and 5 (3 females+2males) DSS+miR-146a_mimics treated miR-146a knock-out mice.
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| Web link |
http://DOI 10.3389/fimmu.2024.1366319
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| Contributor(s) |
Zhu F, Yang T, Peng C |
| Citation(s) |
38799464 |
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| Submission date |
Nov 09, 2023 |
| Last update date |
Jun 05, 2024 |
| Contact name |
Changgeng Peng |
| E-mail(s) |
[email protected]
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| Organization name |
Tongji University
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| Department |
School of Medicine
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| Street address |
Siping Road 1239
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| City |
Yangpu |
| State/province |
Shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (20)
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| GSM7889885 |
DSS treated, WT1 |
| GSM7889886 |
DSS treated, WT2 |
| GSM7889887 |
DSS treated, WT3 |
| GSM7889888 |
DSS treated, miR-146a-/- 1 |
| GSM7889889 |
DSS treated, miR-146a-/- 2 |
| GSM7889890 |
DSS treated, miR-146a-/- 3 |
| GSM7889891 |
DSS+Saline treated, miR-146a-/- 1 |
| GSM7889892 |
DSS+Saline treated, miR-146a-/- 2 |
| GSM7889893 |
DSS+Saline treated, miR-146a-/- 3 |
| GSM7889894 |
DSS+Saline treated, miR-146a-/- 4 |
| GSM7889895 |
DSS+Saline treated, miR-146a-/- 5 |
| GSM7889896 |
DSS+Saline treated, miR-146a-/- 6 |
| GSM7889897 |
DSS+miR-146a mimics treated, miR-146a-/- 1 |
| GSM7889898 |
DSS+miR-146a mimics treated, miR-146a-/- 2 |
| GSM7889899 |
DSS+miR-146a mimics treated, miR-146a-/- 3 |
| GSM7889900 |
DSS+miR-146a mimics treated, miR-146a-/- 4 |
| GSM7889901 |
DSS+miR-146a mimics treated, miR-146a-/- 5 |
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| Relations |
| BioProject |
PRJNA1037549 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE247433_FPKM._WT_and_WT+DSS_and_miR-146a_KO+DSS.xlsx |
11.0 Mb |
(ftp)(http) |
XLSX |
| GSE247433_FPKM._miR-146a_KO+DSS+saline_and_miR-146a_KO+DSS+mimics.xlsx |
5.5 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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