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| Status |
Public on Nov 20, 2024 |
| Title |
CHIP-seq for NKX2-3 overexpressing tumor pericytes |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
This study aims to investigate genes regulated by NKX2-3
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| Overall design |
The tumor pericytes were infected with lentiviruses carrying vector or NKX2-3 overexpression plasmids. Essentially, cells were crosslinked with formaldehyde. After chromatin sonification and centrifugation, 2% of the digested chromatin was saved and the remaining chromatin was equally divided into two aliquots, one for immunoprecipitation by anti-NKX2-3 antibody (HPA047561, Sigma, Shanghai, China), and other as the negative control by adding anti-IgG. After elution from antibody/protein G magnetic beads and reversing the cross-links, the immunoprecipitated DNA was purified and purified for sequencing.
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| Contributor(s) |
Zhang D, Chen M, Yan S, Li X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Nov 22, 2023 |
| Last update date |
Nov 20, 2024 |
| Contact name |
Qun Miao |
| E-mail(s) |
[email protected]
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| Organization name |
Jinan University
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| Street address |
Huangpu avenue
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| City |
Guang Zhou |
| ZIP/Postal code |
510000 |
| Country |
China |
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| Platforms (1) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1044133 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE248500_NKX_annotation.xls.gz |
397.8 Kb |
(ftp)(http) |
XLS |
| GSE248500_RAW.tar |
273.6 Mb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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