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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 06, 2010 |
| Title |
Gene expression change induced by bisphenol A in mouse urogenital sinus |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Bisphenol A (BPA), an endocrine-disrupting chemical (EDC), is a well-known, ubiquitous estrogenic chemical. To investigate the effects of fetal exposure to low-dose BPA on the development of the prostate, we first examined the alterations of in situ sex steroid hormonal environment in the mouse urogenital sinus (UGS).
Next, to investigate the BPA-specific gene alterations related to increases of the E2 levels and aromatase activity, we performed comprehensive gene expression analysis using Affymetrix GeneChip in the BPA-treated or DES-treated male UGS at embryonic day 17th and postnatal day 1st.
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| Overall design |
Pregnant female C57BL/6 mice were exposed to BPA (20 μg/kg/day) or synthetic estrogen Diethylstilbestrol (DES: 0.2 μg/kg/day), which were dissolved in tocopherol-stripped corn oil, on embryonic day 13 (E13) to E16. Between E17 and postnatal day 1 (P1), all animals were terminated by an overdose of isoflurane followed by cervical dislocation. Fetuses were collected at E17, E18, P0, and P1. The bladder and urethra were removed and dissected to isolate UGS and collected in RNA later. To isolate pure UGS, other tissues such as the bladder, urethra, Wolffian duct (WD), seminal vesicle (SV), and Mullerian duct (MD) were removed from both the male and female urogenital tracts. The histopathology of the mouse UGS was then examined by hematoxylin and eosin staining. Total RNA was extracted using the Qiagen mini RNA Easy kit. Each RNAs were linearly amplified and hybridized to Affymetrix GeneChip.
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| Contributor(s) |
Ishii K, Igarashi K |
| Citation(s) |
21123812 |
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| Submission date |
Oct 26, 2010 |
| Last update date |
Feb 11, 2019 |
| Contact name |
KATSUHIDE IGARASHI |
| E-mail(s) |
[email protected]
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| Phone |
+81-3-3786-1011
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| Organization name |
Hoshi University School of Pharmacy and Pharmaceutical Science
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| Department |
Life Science Tokyo Advanced Research center (L-StaR)
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| Street address |
2-4-41 Ebara
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| City |
Shinagawa-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
142-8501 |
| Country |
Japan |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (9)
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| GSM612931 |
UGS at E17 from vehicle exposed fetus |
| GSM612932 |
UGS at E18 from vehicle exposed fetus |
| GSM612933 |
UGS at P0 from vehicle exposed fetus |
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| Relations |
| BioProject |
PRJNA131917 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE24928_RAW.tar |
156.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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