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| Status |
Public on Nov 08, 2024 |
| Title |
METTL14-mediated m6A differentially orchestrates brown and white adipose tissue transcriptomes to regulate systemic metabolism |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
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| Summary |
Human brown and white adipocytes (hBAT and hWAT) display markedly distinct m6A landscapes; besides, in insulin-resistant humans and mice, methyltransferase like 14 (METTL14) expression differs significantly between BAT and WAT in the context of its correlation with insulin sensitivity. We therefore employed independent BAT- and WAT-specific METTL14 knockout models to unveil the cell-type specificity of m6A mRNA methylation. Mettl14 knockout via Ucp1-cre or Adipoq-cre drivers in BAT and WAT, respectively, yields divergent metabolic outcomes in mouse.
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| Overall design |
Mettl14fl/fl mice on a C57BL/6N background (De Jesus et al. 2019) were crossed with Ucp1cre strain (stock no.024670, the Jackson Laboratory) or Adipoqcre strain (stock no.028020, the Jackson Laboratory) mice, which do not harbor the nicotinamide nucleotide transhydrogenase (Nnt) mutation, to generate the knockouts.
For studies on diet-induced obesity and long-term insulin resistance, M14fl/fl,M14fl/fl-Ucp1cre, and M14fl/fl-Adipoqcre mice were fed either 10% fat LFD or a conventional 60% fat HFD for 14 weeks, starting at 6 weeks of age. To examine tissue-specific insulin sensitivity, mice were fasted for 16 hours.
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| Contributor(s) |
Ju C, Xiao L, Kulkarni RN, He C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Dec 13, 2023 |
| Last update date |
Nov 08, 2024 |
| Contact name |
Cheng-Wei Ju |
| E-mail(s) |
[email protected]
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| Organization name |
The University of Chicago
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| Lab |
Chuan He Lab
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| Street address |
929 East 57th Street, GCIS, Room E313
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| City |
Chicago |
| State/province |
Illinois |
| ZIP/Postal code |
60637 |
| Country |
USA |
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| Platforms (2) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (58)
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| GSM7974417 |
human brown adipocyte cell line, Control, input, biol rep 1 |
| GSM7974418 |
human brown adipocyte cell line, Control, input, biol rep 2 |
| GSM7974419 |
human brown adipocyte cell line, Control, input, biol rep 3 |
| GSM7974420 |
human brown adipocyte cell line, Mettl14 KO, input, biol rep 1 |
| GSM7974421 |
human brown adipocyte cell line, Mettl14 KO, input, biol rep 2 |
| GSM7974422 |
human brown adipocyte cell line, Control, m6A IP, biol rep 1 |
| GSM7974423 |
human brown adipocyte cell line, Control, m6A IP, biol rep 2 |
| GSM7974424 |
human brown adipocyte cell line, Control, m6A IP, biol rep 3 |
| GSM7974425 |
human brown adipocyte cell line, Mettl14 KO, m6A IP, biol rep 1 |
| GSM7974426 |
human brown adipocyte cell line, Mettl14 KO, m6A IP, biol rep 2 |
| GSM7974427 |
human white adipocyte cell line, Control, input, biol rep 1 |
| GSM7974428 |
human white adipocyte cell line, Control, input, biol rep 2 |
| GSM7974429 |
human white adipocyte cell line, Control, input, biol rep 3 |
| GSM7974430 |
human white adipocyte cell line, Mettl14 KO, input, biol rep 1 |
| GSM7974431 |
human white adipocyte cell line, Mettl14 KO, input, biol rep 2 |
| GSM7974432 |
human white adipocyte cell line, Mettl14 KO, input, biol rep 3 |
| GSM7974433 |
human white adipocyte cell line, Mettl14 KO, input, biol rep 4 |
| GSM7974434 |
human white adipocyte cell line, Control, m6A IP, biol rep 1 |
| GSM7974435 |
human white adipocyte cell line, Control, m6A IP, biol rep 2 |
| GSM7974436 |
human white adipocyte cell line, Control, m6A IP, biol rep 3 |
| GSM7974437 |
human white adipocyte cell line, Mettl14 KO, m6A IP, biol rep 1 |
| GSM7974438 |
human white adipocyte cell line, Mettl14 KO, m6A IP, biol rep 2 |
| GSM7974439 |
human white adipocyte cell line, Mettl14 KO, m6A IP, biol rep 3 |
| GSM7974440 |
human white adipocyte cell line, Mettl14 KO, m6A IP, biol rep 4 |
| GSM7974441 |
human white adipocyte cell line, Mettl14 KD, input, biol rep 1 |
| GSM7974442 |
human white adipocyte cell line, Mettl14 KD, input, biol rep 2 |
| GSM7974443 |
human white adipocyte cell line, Mettl14 KD, input, biol rep 3 |
| GSM7974444 |
human white adipocyte cell line, Mettl14 KD, m6A IP, biol rep 1 |
| GSM7974445 |
human white adipocyte cell line, Mettl14 KD, m6A IP, biol rep 2 |
| GSM7974446 |
human white adipocyte cell line, Mettl14 KD, m6A IP, biol rep 3 |
| GSM7974447 |
homozygous male mouse, brown adipocytes, Control, input, biol rep 1 |
| GSM7974448 |
homozygous male mouse, brown adipocytes Control, input, biol rep 2 |
| GSM7974449 |
homozygous male mouse, brown adipocytes Control, input, biol rep 3 |
| GSM7974450 |
homozygous male mouse, brown adipocytes Control, input, biol rep 4 |
| GSM7974451 |
homozygous male mouse, brown adipocytes Mettl14 KO, input, biol rep 1 |
| GSM7974452 |
homozygous male mouse, brown adipocytes Mettl14 KO, input, biol rep 2 |
| GSM7974453 |
homozygous male mouse, brown adipocytes Mettl14 KO, input, biol rep 3 |
| GSM7974454 |
homozygous male mouse, brown adipocytes Mettl14 KO, input, biol rep 4 |
| GSM7974455 |
homozygous male mouse, brown adipocytes Control, m6A IP, biol rep 1 |
| GSM7974456 |
homozygous male mouse, brown adipocytes Control, m6A IP, biol rep 2 |
| GSM7974457 |
homozygous male mouse, brown adipocytes Control, m6A IP, biol rep 3 |
| GSM7974458 |
homozygous male mouse, brown adipocytes Control, m6A IP, biol rep 4 |
| GSM7974459 |
homozygous male mouse, brown adipocytes Mettl14 KO, m6A IP, biol rep 1 |
| GSM7974460 |
homozygous male mouse, brown adipocytes Mettl14 KO, m6A IP, biol rep 2 |
| GSM7974461 |
homozygous male mouse, brown adipocytes Mettl14 KO, m6A IP, biol rep 3 |
| GSM7974462 |
homozygous male mouse, brown adipocytes Mettl14 KO, m6A IP, biol rep 4 |
| GSM7974463 |
homozygous male mouse, white adipocytes, Control, input, biol rep 1 |
| GSM7974464 |
homozygous male mouse, white adipocytes Control, input, biol rep 2 |
| GSM7974465 |
homozygous male mouse, white adipocytes Control, input, biol rep 3 |
| GSM7974466 |
homozygous male mouse, white adipocytes Control, input, biol rep 4 |
| GSM7974467 |
homozygous male mouse, white adipocytes Mettl14 KO, input, biol rep 1 |
| GSM7974468 |
homozygous male mouse, white adipocytes Mettl14 KO, input, biol rep 2 |
| GSM7974469 |
homozygous male mouse, white adipocytes Control, m6A IP, biol rep 1 |
| GSM7974470 |
homozygous male mouse, white adipocytes Control, m6A IP, biol rep 2 |
| GSM7974471 |
homozygous male mouse, white adipocytes Control, m6A IP, biol rep 3 |
| GSM7974472 |
homozygous male mouse, white adipocytes Control, m6A IP, biol rep 4 |
| GSM7974473 |
homozygous male mouse, white adipocytes Mettl14 KO, m6A IP, biol rep 1 |
| GSM7974474 |
homozygous male mouse, white adipocytes Mettl14 KO, m6A IP, biol rep 2 |
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| Relations |
| BioProject |
PRJNA1052092 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE250137_M14.iBAT.Ucp1.Deseq2.csv.gz |
2.5 Mb |
(ftp)(http) |
CSV |
| GSE250137_M14.iBAT.Ucp1.RADAR.csv.gz |
488.2 Kb |
(ftp)(http) |
CSV |
| GSE250137_M14.iWAT.Adipoq.Deseq2.csv.gz |
2.4 Mb |
(ftp)(http) |
CSV |
| GSE250137_M14.iWAT.Adipoq.RADAR.csv.gz |
813.9 Kb |
(ftp)(http) |
CSV |
| GSE250137_hBAT.Deseq2.csv.gz |
2.2 Mb |
(ftp)(http) |
CSV |
| GSE250137_hBAT.RADAR.csv.gz |
45.3 Kb |
(ftp)(http) |
CSV |
| GSE250137_hWAT.Deseq2.csv.gz |
2.6 Mb |
(ftp)(http) |
CSV |
| GSE250137_hWAT.RADAR.csv.gz |
128.2 Kb |
(ftp)(http) |
CSV |
| GSE250137_hWAT.siKD.Deseq2.csv.gz |
2.4 Mb |
(ftp)(http) |
CSV |
| GSE250137_hWAT.siKD.RADAR.csv.gz |
85.3 Kb |
(ftp)(http) |
CSV |
| GSE250137_hWAT_hBAT.Deseq2.csv.gz |
2.5 Mb |
(ftp)(http) |
CSV |
| GSE250137_hWAT_hBAT.RADAR.csv.gz |
651.8 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
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