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Series GSE25238 Query DataSets for GSE25238
Status Public on Nov 10, 2010
Title Gene expression profile associated with p53R2 in human cancer cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Deregulation of the expression of p53R2, a p53-inducible homologue of the R2 subunit of ribonucleotide reductase, has been found in various human cancer tissues; however, the roles p53R2 plays in cancer progression and malignancy remain controversial. In present design, we aimed to identify changes in gene expression profiles associated with p53R2 in cancer cells. We compared gene expression file of control KB cells with that of p53R2-attenuated KB cells by its specific siRNA by using cDNA microarray technology.
We used microarrays to display the global gene expression profile change, after knockdown of p53R2 expression in human oropharyngeal carcinoma KB cells, to find the gene set regulated by p53R2.
 
Overall design To compare changes in gene expression profile in human oropharyngea carcinoma KB cells, KB cells were transfected with control siRNA or p53R2 siRNA, 48h later, total RNA and protein were subjected to Q-PCR and Western blot to confirm a significantly decreased p53R2 mRNA and protein, and then these total RNA samples were for microarray analysis. The cDNA microarray data was analyzed to explore gene sets/pathways, which were significantly impacted by knockdown of p53R2.
 
Contributor(s) Zhang K, Wu X, Yen Y
Citation(s) 21216934
Submission date Nov 09, 2010
Last update date Jul 26, 2018
Contact name keqiang zhang
Organization name City of Hope National Medical Center
Department Molecular Pharmacology
Street address 1500 East Duarte Road
City Duarte
State/province CA
ZIP/Postal code 91010
Country USA
 
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (4)
GSM621124 Control siRNA 1
GSM621125 Control siRNA 2
GSM621126 p53r2 siRNA 1
Relations
BioProject PRJNA134903

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Supplementary file Size Download File type/resource
GSE25238_RAW.tar 16.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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