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| Status |
Public on Nov 06, 2016 |
| Title |
KYSE (450, 520, 890, 960) cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
microRNA profiles in human esophageal squamous cell carcinoma cell lines
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| Overall design |
KYSE cell lines were maintained in Ham’s F-12/RPMI 1640 (Invitrogen, San Diego, CA) containing 5% fetal bovine serum at 37 ˚C in a fully-humidified 5% CO2 atmosphere. Total RNA was extracted by the acid guanidinium thiocyanate-phenol-chloroform method. According to manufacture’s instructions, 500 ng of total RNA was labeled with Hy5 and was hybridized on the Human miRNA Oligo chip for 16 hour at 32˚C. A Human miRNA Oligo chip was scanned using ProScanArray (PerkinElmer).
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| Contributor(s) |
Tsuchiya S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 18, 2010 |
| Last update date |
Nov 06, 2016 |
| Contact name |
Soken Tsuchiya |
| Organization name |
Kumamoto University
|
| Street address |
5-1 Oe-honmachi, Chuo-ku
|
| City |
Kumamoto |
| ZIP/Postal code |
862-0973 |
| Country |
Japan |
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| Platforms (1) |
| GPL10312 |
3D-Gene Human miRNA Oligo chip v12-1.00 |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA134601 |
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