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| Status |
Public on Feb 29, 2024 |
| Title |
FOXO1 is a master regulator of CAR T memory programming [ATAC_FOXO1] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Poor CAR T persistence limits CAR T cell therapies for B cell malignancies and solid tumors1,2. The expression of memory-associated genes such as TCF7 (protein name TCF1) is linked to response and long-term persistence in patients3–7, thereby implicating memory programs in therapeutic efficacy. Here, we demonstrate that the pioneer transcription factor, FOXO1, is responsible for promoting memory programs and restraining exhaustion in human CAR T cells. Pharmacologic inhibition or gene editing of endogenous FOXO1 in human CAR T cells diminished the expression of memory-associated genes, promoted an exhaustion-like phenotype, and impaired antitumor activity in vitro and in vivo. FOXO1 overexpression induced a gene expression program consistent with T cell memory and increased chromatin accessibility at FOXO1 binding motifs. FOXO1-overexpressing cells retained function, memory potential, and metabolic fitness during settings of chronic stimulation and exhibited enhanced persistence and antitumor activity in vivo. In contrast, TCF1 overexpression failed to enforce canonical memory programs or enhance CAR T cell potency. Importantly, endogenous FOXO1 activity correlated with CAR T and TIL responses in patients, underscoring its clinical relevance in cancer immunotherapy. Our results demonstrate that memory reprogramming through FOXO1 can enhance the persistence and potency of human CAR T cells and highlights the utility of pioneer factors, which bind condensed chromatin and induce local epigenetic remodeling, for optimizing therapeutic T cell states.
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| Overall design |
Epigenetic study of HA.28z CAR T cells with FOXO1 (wild type), FOXO1-DBDmut (K245>A, K248>A), or NGFR empty vector transcription factors over expressed to evaluate epigenetic remodeling activity of a FOXO1 mutant that has lower affinity for DNA binding
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| Contributor(s) |
Doan A, Mueller K, Chen A, Rouin G, Weber E |
| Citation(s) |
38600391 |
| |
| Submission date |
Feb 08, 2024 |
| Last update date |
May 09, 2024 |
| Contact name |
Katherine Paige Mueller |
| E-mail(s) |
[email protected]
|
| Phone |
3037758187
|
| Organization name |
Children's Hospital of Philadelphia
|
| Department |
Pediatrics
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| Lab |
Weber Lab
|
| Street address |
3501 Civic Center Blvd
|
| City |
Philadelphia |
| State/province |
Pennsylvania |
| ZIP/Postal code |
19104-3820 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE255416 |
FOXO1 is a master regulator of CAR T memory programming |
|
| Relations |
| BioProject |
PRJNA1074820 |
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