|
| Status |
Public on Sep 15, 2024 |
| Title |
129-derived passenger mutations confound phenotype of ACKR1-deficient mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
A majority of genetically modified mice carry passenger mutations, originating from the 129-derived embryonic stem (ES) cells, near the targeted gene. Unintended retention of these mutations can introduce confounding phenotypes and affect conclusions.
We show that Ackr1-/-129ES mice retained approximately 6 Mb of 129 ES cells-derived genomic material in chromosome 1 near Ackr1 in bone marrow derived monocytes (Mono), nucleated erythroblasts (NECs), and polymorphonucleated neutrophils (PMN), notably affecting the expression of PYHIN genes.
|
| |
|
| Overall design |
C57BL/J (n = 3, WT CTRL) and ACKR1-deficient mice (n = 3, KO); no technical replicates
|
| Web link |
https://academic.oup.com/jleukbio/advance-article/doi/10.1093/jleuko/qiae208/7774820?login=false
|
| |
|
| Contributor(s) |
Duchene J, Möller-Ramon Z |
| Citation(s) |
39319406 |
| |
| Submission date |
Feb 16, 2024 |
| Last update date |
Oct 04, 2024 |
| Contact name |
Johan Duchene |
| E-mail(s) |
[email protected], [email protected]
|
| Phone |
+4989440054757
|
| Organization name |
LMU
|
| Department |
IPEK
|
| Street address |
Pettenkoferstrasse, 9
|
| City |
Munich |
| State/province |
- Please Select - |
| ZIP/Postal code |
80336 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL20775 |
[MTA-1_0] Affymetrix Mouse Transcriptome Array 1.0 [transcript (gene) CSV version] |
|
| Samples (18)
|
|
| Relations |
| BioProject |
PRJNA1077396 |
Less...
More...