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Series GSE2585

Query DataSets for GSE2585

Status

Public on Apr 28, 2005

Title

promiscuous gene expression in the mouse thymus

Organism

Mus musculus

Experiment type

Expression profiling by array

Summary

Gene expression in different thymic stromal cells and subsets thereof was analyzed in 6-12 week old wild type (C57BL/6) and Aire knock-out (mixed background) mice. Thymic stromal cells were purified by sequential enzymatic digestion (collagenase, collagenase/dispase and trypsin) followed by gradient centrifugation and FACS sorting. Sort criteria were as follows: dendritic cells (CD11c+, F4/80 -), macrophages (F4/80+, CD11c-), cTECs (CD45–/lo, CDR1/Ly51+, Ep-CAM+) and mTECs (CD45–/lo, CDR1/Ly51–, Ep-CAM+). mTECs of wild-type and Aire knock-out mice were further subdivided according to CD80 expression levels.
For microarray analysis total RNA from thymic stromal cell samples of two independent experiments was pre-amplified and biotinylated by two rounds of cDNA synthesis and in vitro transcription. Fluorescence readings were evaluated by using Microarray Suite 5.0 software.
Keywords = thymus
Keywords = promiscuous gene expression
Keywords = AIRE
Keywords: ordered

Contributor(s)

Derbinski J, Gaebler J, Brors B, Tierling S, Jonnakuty S, Hergenhahn M, Peltonen L, Walter J, Kyewski B

Citation(s)

15983066

Submission date

Apr 27, 2005

Last update date

Feb 18, 2018

Contact name

Benedikt Brors

E-mail(s)

[email protected]