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Series GSE26207 Query DataSets for GSE26207
Status Public on Jul 14, 2011
Title The roles of ppar-delta and ppar-gamma in regulating the metabolic switch between oxidation and storage of fats in white adipose tissue
Organism Mus musculus
Experiment type Expression profiling by array
Summary Obesity is a major risk factor for the development of insulin resistance and type II diabetes. The nuclear receptors PPAR-delta and PPAR-gamma play a central role in regulating metabolism in adipose tissue, as well as being targets for the treatment of insulin resistance. The metabolic effects of PPAR-delta and PPAR-gamma activation have been examined both in vivo in white adipose tissue from ob/ob mice and in vitro in cultured 3T3-L1 adipocytes using a combined 1H NMR spectroscopy and mass spectrometry metabolomic methodology to understand the contrasting roles of these receptors. These steady state measurements were supplemented with 13C-stable isotope substrate labeling to assess fluxes, respirometry and transcriptomic microarray analysis. The metabolic effects of the two receptors were readily distinguished, with PPAR-gamma activation characterised by increased fat storage and fat synthesis/elongation, while activation of PPAR-delta caused increased fatty acid beta-oxidation, TCA cycle rate and oxidation of extracellular branch chain amino acids. Stimulated glycolysis and increased desaturation of fatty acids were the only common pathways. PPAR-delta has a role as an anti-obesity target as well as an anti-diabetic.
 
Overall design Total RNA obtained from cultured 3T3-L1 cells treated for 48 hours with either DMSO control, GW610742 PPARd agonist or GW347845 PPARg agonist and compared.
 
Contributor(s) Roberts LD, Murray AJ, Menassa D, Ashmore T, Nicholls AW, Griffin JL
Citation(s) 21843327
Submission date Dec 20, 2010
Last update date Jan 16, 2019
Contact name Julian Griffin
E-mail(s) [email protected]
Phone +44(0)1223 333626
Organization name University of Cambridge
Department Biochemistry
Lab Griffin
Street address Tennis Court Road
City Cambridge
ZIP/Postal code CB2 1QW
Country United Kingdom
 
Platforms (1)
GPL6887 Illumina MouseWG-6 v2.0 expression beadchip
Samples (24)
GSM643492 3T3-L1-DMSO Control-1 (delta normalized)
GSM643493 3T3-L1-DMSO Control-2 (delta normalized)
GSM643494 3T3-L1-DMSO Control-3 (delta normalized)
Relations
BioProject PRJNA135035

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26207_RAW.tar 15.8 Mb (http)(custom) TAR
GSE26207_non-normalized.txt.gz 11.9 Mb (ftp)(http) TXT
Processed data included within Sample table
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