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| Status |
Public on May 08, 2024 |
| Title |
FOXO1 enhances CAR T cell stemness, metabolic fitness and efficacy [human_FOXO1OE_stimulation/rest_RNAseq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Investigating the transcriptional profile of human FOXO1 overexpressing α-lewisY CAR T cells after stimulation or after stimulation and rest
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| |
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| Overall design |
Human anti-Lewis Y CAR T cells overexpressing FOXO1 or mCherry (control) were generated from healthy donor peripheral blood mononuclear cells (PBMC).Control or FOXO1- expressing CD8+ CAR T cells were assessed by RNA-Sequencing prior to stimulation, after 24 h activation with 0.1 μg/ml plate bound anti-Lewis Y or after 7 days recovery post stimulation with anti-Lewis Y.
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| |
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| Contributor(s) |
Beavis P, Darcy P, Sek K, Munoz I |
| Citation(s) |
38600376 |
| |
| Submission date |
Apr 04, 2024 |
| Last update date |
May 08, 2024 |
| Contact name |
Kevin C sek |
| E-mail(s) |
[email protected]
|
| Phone |
0432960331
|
| Organization name |
Peter MacCallum Cancer Centre
|
| Department |
Cancer Immunology
|
| Lab |
Beavis Lab
|
| Street address |
305 Grattan Street
|
| City |
Melbourne |
| State/province |
VIC |
| ZIP/Postal code |
3000 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE225527 |
Overexpression of FOXO1 enhances CAR T cell polyfunctionality, metabolic fitness and efficacy against solid tumors |
|
| Relations |
| BioProject |
PRJNA1096359 |
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