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| Status |
Public on Jun 01, 2024 |
| Title |
Deciphering Mycobacterium tuberculosis glycan epitopes through clustering of human polyclonal and isolating novel monoclonal antibodies I |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Protein profiling by protein array
|
| Summary |
The immunomodulatory mycobacterial surface antigen lipoarabinomannan (LAM) with its immunogenic glycan component arabinomannan (AM) facilitates Mycobacterium tuberculosis’ (Mtb) escape from the host’s immune response. Some but not all antibodies against AM can protect against TB. To better understand which of AM’s structures to target, we must first identify the spectrum of its epitopes.
We here used synthetic AM oligosaccharide motifs to deplete sera from subjects along the spectrum of Mtb infection. Performing cluster analysis, we were able to predict AM’s immunogenic structures and delineate how antibody reactivity to them is influenced by Mtb infection states.
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| Overall design |
Human plasma samples were tested for direct binding to 30 synthetic oligosaccharides
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| Contributor(s) |
Chen T, Liu Y, Lowary TL, Achkar JM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 08, 2024 |
| Last update date |
Jun 01, 2024 |
| Contact name |
Jacqueline Achkar |
| E-mail(s) |
[email protected]
|
| Organization name |
Albert Einstein College of Medicine
|
| Street address |
1300 Morris Park Ave
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
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| Platforms (1) |
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| Samples (107)
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| Relations |
| BioProject |
PRJNA1097647 |
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