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| Status |
Public on Oct 30, 2024 |
| Title |
Global transcriptomic alterations in MDA-MB-231 cells following high salt treatment |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Difference in salt concentration may cause dramatic alterations in tumor microenvironment, progression of cancer and overall outcome of cancer therapy. Immune cells in tumor microenvironment are mostly immunosuppressed under the influence of tumor cells. Recent studies has shown that high salt induces immune cells to pro-inflammatory state in the tumor microenvironment. On contrary, it hinders the immunosuppressive functions of regulatory T cells, which plays an important role in inhibiting anti-cancer immune responses in tumor microenvironment. Salt- mediated induction of the immune cells to pro-inflammatory state could potentially enhance their transition from the immunosuppressed state to their innate pro- inflammatory state, thereby facilitating their effectiveness in combating the tumor and suppressing tumor progression. The role of high salt in cancer is controversial with only a few studies suggesting the potential pro-tumorigenic effect of high salt. Dietary salt increased the risk of gastric cancer, contributing to both the incidence and mortality of the disease by damaging the gastric mucosal lining. Another research indicated that a high salt diet could stimulate the growth of breast cancer and promote lung metastasis by elevating Th17 cell levels. In contrast, high salt containing diets significantly inhibited tumor growth, primarily through the depletion and functional modulation of myeloid-derived suppressor cells (MDSCs), highlighting its anti-cancer efficacy. High salt reduced cytokine production necessary for the expansion of myeloid-derived suppressor cells (MDSCs) and decreases MDSC accumulation in blood, spleen, and tumors. High salt diet in mice also showed increased Bifidobacterium abundance and heightened gut permeability, leading to intratumor localization of Bifidobacterium and enhancing NK cell functions. This facilitated tumor regression, suggesting potential role of high salt in cancer immunotherapy. The overall impact of high-salt diets is not fully understood, which necessitates further research to elucidate their influence in altering tumor microenvironment and fate of cancer cells. This study aimed to investigate the effects of high salt on a triple-negative breast cancer cell line (in vitro), exploring its impact on cancer progression and associated global transcriptomic changes.
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| Overall design |
To study the effect of high salt in triple negative breast cancer cell line (MDA MB-231), control (1 Sample) and high salt (2 Replicates) treated cell line were subjected to RNA Seq analysis. Total RNA was extracted and subjected to RNA Seq analysis.The impact of high salt on breast cancer cells has not been previously explored. Therefore, in this study, we investigated the influence of high salt on the triple-negative MDA MB-231 in-vitro.
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| Web link |
https://link.springer.com/article/10.1007/s11033-024-09925-4
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| Contributor(s) |
Sharma M, Dey U, Olymon K, Kumar A, Mukhopadhyay R |
| Citation(s) |
39305332 |
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| Submission date |
Apr 26, 2024 |
| Last update date |
Oct 30, 2024 |
| Contact name |
Aditya Kumar |
| E-mail(s) |
[email protected]
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| Organization name |
Tezpur University
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| Department |
Molecular Biology and Biotechnology
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| Lab |
Nucleix Lab
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| Street address |
R107, Dept Of Molecular Biology & Biotechnology
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| City |
Tezpur |
| State/province |
Assam |
| ZIP/Postal code |
784028 |
| Country |
India |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA1104995 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE265934_count_matrix.txt.gz |
852.6 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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