| Overall design |
We recorded the embryonic development process of Meloidogyne incognita, Heterodera glycines, and Ditylenchus destructor species, and constructed transcriptome datasets of single embryos of these three species utilized low-input smart-seq2 RNA-seq technology. The datasets encompassed 11 complete embryonic development stages, which are Zygote, 2-cell, 4-cell, 8-cell, 24-44 cell, 64-78 cell, Comma, 1.5-fold, 2-fold, Moving, L1, which each stage generated four to five replicates. a total of 162 high-resolution transcriptome libraries were obtained.
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