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| Status |
Public on Nov 21, 2024 |
| Title |
Transcriptomic analysis of coxsackievirus B3 infection in induced pluripotent stem cell-derived brain-like endothelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
ABSTRACT: Viral aseptic meningitis is a neuroinflammatory condition that occurs when viruses gain access to the central nervous system (CNS) and induce inflammation. The blood–brain barrier (BBB) is comprised of brain endothelial cells (BECs) that stringently regulate the passage of molecules, toxins, and pathogens from the circulation into the CNS. Through their unique properties, such as complex tight junctions, reduced rates of endocytosis, expression of efflux transporters, and restricted expression of leukocyte adhesion molecules, the BBB is often able to limit pathogen entry into the brain; however, certain neurotropic pathogens, such as coxsackievirus B3 (CVB3) are able to infect the CNS. We have previously demonstrated that CVB3 can infect and disrupt induced pluripotent stem cell-derived brain-like endothelial cells (iBECs), but the host response to this infection remains unknown. Here, we investigate global
host transcriptional changes during CVB3 infection of iBECs using RNA sequencing. We validated our data set by exploring pathways altered by CVB3 using quantitative real-time PCR (qPCR) and enzyme-linked immunosorbent assay of upregulated cytokines
and interferon signaling molecules.
IMPORTANCE: Coxsackievirus B3 (CVB3) is a leading cause of viral aseptic meningitis that can cause severe disease in susceptible individuals. To gain access to the central nervous system, CVB3 must cross central nervous system barriers, such as the blood–
brain barrier. Previously, we have shown that CVB3 infects a human stem cell-derived brain-like endothelial cell model. Here, we report the global transcriptome of stem cell-derived brain-like endothelial cells to CVB3 infection and provide proof-of-concept validation of the dataset using molecular biology techniques. These data could inform novel mechanisms of CVB3-mediated blood–brain barrier dysfunction.
KEYWORDS: Coxsackievirus B3, blood-brain barrier, brain endothelial cells, RNA sequencing, induced pluripotent stem cells
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| Overall design |
To investigate Coxsackievirus B3 infection (after 2 days and 5 days) on human pluripotent stem-cell derived brain-like endothelial cells using transcriptomics (RNA seq) with differential expression analyses, qPCR, and ELISA.
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| Contributor(s) |
Hathcock SF, Mamana J, Keyzer TE, Vollmuth N, Shokri M, Mauser HD, Correll RN, Lam DW, Kim BJ, Sin J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R15 NS131921 |
Impairment of the cerebral vasculature during bacterial meningitis |
THE UNIVERSITY OF ALABAMA |
Brandon Jonathan Kim |
| R01 DK125692 |
Targeting transient receptor potential channels to suppress proviral mitochondrial fission and mitophagy in order to mitigate CVB pancreatitis |
THE UNIVERSITY OF ALABAMA |
Jon Sin |
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| Submission date |
Jun 07, 2024 |
| Last update date |
Nov 22, 2024 |
| Contact name |
Daryl Lam |
| E-mail(s) |
[email protected]
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| Organization name |
University of Alabama
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| Department |
Biological Sciences
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| Street address |
Box 870344
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| City |
Tuscaloosa |
| State/province |
Alabama |
| ZIP/Postal code |
35487 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (12)
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| GSM8314911 |
Coxsackievirus infection at 2 day (replicate 1 of 3) |
| GSM8314912 |
Coxsackievirus infection at 2 day (replicate 2 of 3) |
| GSM8314913 |
Coxsackievirus infection at 2 day (replicate 3 of 3) |
| GSM8314914 |
Coxsackievirus infection at 5 day (replicate 1 of 3) |
| GSM8314915 |
Coxsackievirus infection at 5 day (replicate 2 of 3) |
| GSM8314916 |
Coxsackievirus infection at 5 day (replicate 3 of 3) |
| GSM8314917 |
Negative control at 2 day (replicate 1 of 3) |
| GSM8314918 |
Negative control at 2 day (replicate 2 of 3) |
| GSM8314919 |
Negative control at 2 day (replicate 3 of 3) |
| GSM8314920 |
Negative control at 5 day (replicate 1 of 3) |
| GSM8314921 |
Negative control at 5 day (replicate 2 of 3) |
| GSM8314922 |
Negative control at 5 day (replicate 3 of 3) |
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| Relations |
| BioProject |
PRJNA1121408 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE269413_2_day_raw.counts.csv.gz |
487.2 Kb |
(ftp)(http) |
CSV |
| GSE269413_5_day_raw_counts.csv.gz |
489.9 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
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