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| Status |
Public on Aug 26, 2011 |
| Title |
The consequences of n-3 polyunsaturated fatty acids (PUFA) depletion on hepatic lipid metabolism in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
In the present study, we investigated the consequences of n-3 polyunsaturated fatty acids (PUFA) depletion on hepatic lipid metabolism in mice fed during three months with a diet presenting a high n-6/n-3 PUFA ratio to induce n-3 PUFA depletion. Microarray analyses were performed to identify the molecular targets involved in the development of hepatic steatosis associated with n-3 PUFA depletion.
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| Overall design |
Male C57Bl/6J mice (9 weeks old) were housed in groups of four mice per cage at 22°C in a 12h light/dark cycle and given free access to diet and water. After an acclimatization period of 1 week, the mice were fed a control (CT) or an n-3 PUFA-depleted diet (DEF) for 3 months. At the end of the study period, mice (CT, n=6; DEF, n=7) were anesthetized after a 6h period of fasting. Some mice (CT: n=4 et DEF: n=7) were anesthetized without being starved. A fraction of the main liver lobe was fixed-frozen in isopentane and kept at -80°C for histological analysis. The excess tissue material was immediately clamped in liquid N2 and kept at -80°C. All mice experiments were approved by the local animal ethics committee, and the housing conditions were as specified by the Belgian Law of November 14, 1993 on the protection of laboratory animals (agreement n° LA 1230314).
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| Contributor(s) |
Pachikian BD, Demoulin J, Essaghir A, Delzenne NM |
| Citation(s) |
21853118 |
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| Submission date |
Jan 31, 2011 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Jean-Baptiste Demoulin |
| Organization name |
Université catholique de Louvain
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| Department |
de Duve Institute
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| Lab |
UCL - MEXP
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| Street address |
av Hippocrate 75, B1.74.05
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| City |
Brussels |
| ZIP/Postal code |
B-1200 |
| Country |
Belgium |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (4)
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| GSM664751 |
Mice fed with control diet and starved before sacrifice |
| GSM664752 |
Mice fed with n-3 PUFA-depleted diet and starved before sacrifice |
| GSM664753 |
Mice fed with control diet |
| GSM664754 |
Mice fed with n-3 PUFA-depleted diet |
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| Relations |
| BioProject |
PRJNA135935 |
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