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Series GSE2705 Query DataSets for GSE2705
Status Public on Aug 05, 2005
Title TGF-beta regulated gene transcription and protein expression in human GFAP-negative lamina cribrosa cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Primary open angle glaucoma (POAG) is a progressive optic neuropathy, which is a major cause of worldwide visual impairment and blindness. Pathological hallmarks of the glaucomatous optic nerve head include retinal ganglion cell axon loss and extracellular matrix (ECM) remodeling of the lamina cribrosa layer. TGF-beta is an important pro-fibrotic modulator of ECM metabolism, whose levels are elevated in human POAG lamina cribrosa tissue compared with non-glaucomatous controls. We treated human lamina cribrosa (LC) cells with TGF-beta1 (10ng/ml) for 24 hours in order to examine differential gene expression patterns in repsonse to this cytokine. In particular we focused on ECM and fibrotic genes. We found that TGF-beta1 induces expression and release of ECM components in LC cells, which may be important in regulating matrix remodeling in the lamina cribrosa. In disease states such as POAG, the LC cell may represent an important pro-fibrotic cell type and an attractive target for novel therapeutic strategies.
Keywords: other
 
 
Contributor(s) Kirwan RP, Leonard MO, Murphy M, Clark AF, O'Brien CJ
Citation(s) 16078232
Submission date May 24, 2005
Last update date Aug 10, 2018
Contact name Ruaidhri Patrick Kirwan
E-mail(s) [email protected]
Organization name University College Dublin
Department Conway Institute of Biomolecular and Biomedical Research & School of Medicine and Medical Science
Lab Dr. Martin Leonard
Street address Belfield
City Dublin
State/province Leinster
ZIP/Postal code D4
Country Ireland
 
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (4)
GSM52088 TGFb1 treated experiment A (10ng/ml 24 hours)
GSM52089 vehicle treated experiment A (4mM HCL/1% BSA) 24 hours
GSM52090 TGFb1 treated experiment B (10ng/ml 24 hours)
Relations
BioProject PRJNA92353

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