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| Status |
Public on Jun 25, 2024 |
| Title |
The effect of constitutive root isoprene emission on root phenotype and physiology under control and salt stress conditions |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Isoprene, a volatile hydrocarbon, is typically emitted from the leaves and other aboveground plant organs; isoprene emission from roots is not well studied. Given its well-known function in plant growth and defense aboveground, isoprene may also be involved in shaping root physiology to resist belowground stress. We used isoprene-emitting transgenic lines (IE) and a non-emitting empty vector and/or wild type lines (NE) of Arabidopsis to elucidate the roles of isoprene in root physiology and salt stress resistance. We assessed root phenotype and metabolic changes, hormone biosynthesis and signaling, and stress-responses under normal and saline conditions of IE and NE lines. We also analyzed the root transcriptome in the presence or absence of salt stress. IE lines emitted isoprene from roots, which was associated with higher primary root growth, root biomass, and root/shoot biomass ratio under both control and salt stress conditions. Transcriptome data indicated that isoprene altered the expression of key genes involved in hormone metabolism and plant responses to stress factors. Our findings reveal that root constitutive isoprene emission sustains root growth also under salinity by regulating and/or priming hormone biosynthesis and signaling mechanisms, amino acids biosynthesis, and expression of key genes relevant to salt stress defense.
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| Overall design |
To investigate the root transcriptome changes in Isoperene overexpression lines compared with Wild-type plants in response to different salinity stress conditions.
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| Web link |
https://www.biorxiv.org/content/10.1101/2024.02.09.579703v1
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| Contributor(s) |
Sharkey T, Abdelrhman M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jun 24, 2024 |
| Last update date |
Jun 25, 2024 |
| Contact name |
Mostafa ABDELWAHED NOURELDEIN ABDELRAHMAN |
| E-mail(s) |
[email protected]
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| Phone |
8068051625
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| Organization name |
TEXAS TECH UNIVERSITY
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| Street address |
3120 4th Street, Lubbock, TX, USA
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| City |
Lubbock |
| State/province |
TX |
| ZIP/Postal code |
79415 |
| Country |
USA |
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| Platforms (1) |
| GPL26208 |
Illumina NovaSeq 6000 (Arabidopsis thaliana) |
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| Samples (18)
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| GSM8345871 |
Root, 4-WT0_S4 |
| GSM8345872 |
Root, 16-C40_S13 |
| GSM8345873 |
Root, 17-C40_S14 |
| GSM8345874 |
Root, 20-C40_S16 |
| GSM8345875 |
Root, 21-WT150_S17 |
| GSM8345876 |
Root, 25-WT150_S20 |
| GSM8345877 |
Root, 36-C4150_S29 |
| GSM8345878 |
Root, 37-C4150_S30 |
| GSM8345879 |
Root, 39-C4150_S31 |
| GSM8345880 |
Root, 12-B20_S9 |
| GSM8345881 |
Root, 14-B20_S11 |
| GSM8345882 |
Root, 15-B20_S12 |
| GSM8345883 |
Root, 31-B2150 |
| GSM8345884 |
Root, 32-B2150 |
| GSM8345885 |
Root, 34-B2150 |
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| Relations |
| BioProject |
PRJNA1127435 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE270516_Processed_data.txt.gz |
2.3 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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