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| Status |
Public on Feb 02, 2014 |
| Title |
Gene Expression Profile of Moraxella catarrhalis Grown in Pooled Human Sputum |
| Platform organism |
Moraxella catarrhalis |
| Sample organism |
Moraxella catarrhalis ATCC 43617 |
| Experiment type |
Expression profiling by array
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| Summary |
Comparison of the gene expression profile of Moraxella catarrhalis grown in the presence of 20% pooled human sputum in chemically-defined medium relative to Moraxella catarrhalis grown in chemically-defined medium alone.
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| Overall design |
Moraxella catarrhalis ATCC43617 was grown to mid-logarithmic phase either in the presence of 20% pooled human sputum in chemically-defined medium or in chemically-defined medium alone. Total RNA was extracted from bacterial cells exposed to each of these conditions and cDNA was generated for CyDye labelling. 3 biologic replicates were generated and each replicate underwent a dye swap (total of 6 experimental data collections). The gene expression profile reported is that of Moraxella catarrhalis grown in the presence of pooled human sputum in a chemically-defined medium relative to Moraxella catarrhalis grown only in the presence of the chemically-defined medium.
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| Contributor(s) |
Murphy T, Brauer A, Hoopman T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 03, 2011 |
| Last update date |
Feb 02, 2014 |
| Contact name |
Todd C. Hoopman |
| E-mail(s) |
[email protected]
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| Phone |
214-648-5971
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| Organization name |
University of Texas Southwestern Medical Center
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| Department |
Microbiology
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| Street address |
5323 Harry Hines Blvd.
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| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390-9048 |
| Country |
USA |
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| Platforms (1) |
| GPL7398 |
EJH Mcat Oligo-DNA Microarray 3 |
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| Samples (6)
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| GSM667815 |
Gene Expression Profile of Moraxella catarrhalis Grown in Pooled Human Sputum (Rep_2A) |
| GSM667816 |
Gene Expression Profile of Moraxella catarrhalis Grown in Pooled Human Sputum (Rep_3A) |
| GSM667817 |
Gene Expression Profile of Moraxella catarrhalis Grown in Pooled Human Sputum (Rep_1A) |
| GSM667818 |
Gene Expression Profile of Moraxella catarrhalis Grown in Pooled Human Sputum (Rep_1B) |
| GSM667827 |
Gene Expression Profile of Moraxella catarrhalis Grown in Pooled Human Sputum (Rep_2B) |
| GSM667862 |
Gene Expression Profile of Moraxella catarrhalis Grown in Pooled Human Sputum (Rep_3B) |
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| Relations |
| BioProject |
PRJNA137619 |
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