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| Status |
Public on Aug 08, 2024 |
| Title |
RNA-seq based transcriptome analysis of plant pathogens under salt stress condition |
| Organisms |
Ralstonia solanacearum; Pectobacterium carotovorum subsp. carotovorum; Burkholderia gladioli |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Plant pathogens require lethal virulence factors, susceptible hosts, and optimal environmental conditions for disease establishment. High soil salinity, exacerbated by climate change, significantly impacts agro-biological ecosystems. However, the overall interactions between plant pathogens and salt stress are not fully characterized or understood. This study examines the effects of salt stress on representative plant pathogens: Burkholderia gladioli, Pectobacterium carotovorum subsp. carotovorum, and Ralstonia solanacearum. Using pan-genome-based comparative transcriptomics, we analyzed the comprehensive alterations within the biological systems of plant pathogens when treated with 200 mM NaCl. Our results highlight the differential responses between salt-sensitive and salt-tolerant pathogens to salt stress.
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| Overall design |
To induce salt stress, 200 mM NaCl was applied to salt-sensitive (B. gladioli and R. solanacearum) and salt-tolerant (Pcc) pathogens. For each plant pathogen, cultures without NaCl addition were used as negative controls. After 2 hours of exposure to salt stress, we examined changes in gene expression patterns. Each sample was prepared in triplicate.
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| Contributor(s) |
Park J, Jung H, Seo Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jul 24, 2024 |
| Last update date |
Aug 09, 2024 |
| Contact name |
Jungwook Park |
| E-mail(s) |
[email protected]
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| Organization name |
Pusan National University
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| Department |
Microbiology
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| Street address |
Pusan National University, 2, Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 609-735, Korea
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| City |
Busan |
| ZIP/Postal code |
ASI|KR|KS012|PUSAN |
| Country |
South Korea |
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| Platforms (3) |
| GPL29295 |
Illumina NovaSeq 6000 (Ralstonia solanacearum) |
| GPL34740 |
Illumina NovaSeq 6000 (Burkholderia gladioli) |
| GPL34741 |
Illumina NovaSeq 6000 (Pectobacterium carotovorum subsp. carotovorum) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA1139749 |
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