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| Status |
Public on Sep 01, 2012 |
| Title |
Expression data from electrically-altered larval Drosophila LNvs |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
While intensely studied in the context of synaptic plasticity, the interplay between electrical activity and transcription is also relevant to circadian pacemaker neurons where ~24hr rhythms in gene expression and neural activity define the functional state of clock neurons. Here we demonstrate broad transcriptional changes in Drosophila circadian pacemaker neurons (LNvs) in response to altered electrical activity, including a large set of circadian genes.
We used microarrays to identify the global program of gene expression in purified Drosophila pacemaker neurons in response to targeted electrical manipulations
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| Overall design |
Drosophila larval brains (L3 stage) were dissected on the second day in constant darkness following a 12:12hr light entrainment regime. Larvae contained a Pdf-RFP transgene that labels 8 LNvs pacemaker neurons per brain, allowing purification by flow cytometry. Larvae also contained transgenes (NachBac and Kir) to specifically manipulate the electrical properties of LNvs. Brains were dissociated and LNvs purified by FACS, followed by RNA amplification and hybridization on Affymetrix microarrays.
We generated expression profiles of LNvs isolated at CT3 expressing Kir and GFP in the cyc0 mutant background. Drosophila larval brains (L3 stage) were dissected on the second day in constant darkness following a 12:12hr light entrainment regime. Brains were dissociated and LNvs purified by FACS, followed by RNA amplification and hybridization on Affymetrix microarrays.
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| Contributor(s) |
Ruben M, Mizrak D, Blau J |
| Citation missing |
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| Submission date |
Feb 28, 2011 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Marc Ruben |
| Organization name |
New York University
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| Department |
Biology
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| Lab |
Justin Blau
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| Street address |
100 Washington Square East
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10003 |
| Country |
USA |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (27)
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| GSM682266 |
larval LNvs at CT-3, biological rep1 |
| GSM682267 |
larval LNvs at CT-3, biological rep2 |
| GSM682268 |
larval LNvs at CT-3, biological rep3 |
| GSM682269 |
larval LNvs at CT-15, biological rep1 |
| GSM682270 |
larval LNvs at CT-15, biological rep2 |
| GSM682271 |
larval LNvs at CT-15, biological rep3 |
| GSM682272 |
larval NCB-LNvs at CT-15, biological rep1 |
| GSM682273 |
larval NCB-LNvs at CT-15, biological rep2 |
| GSM682274 |
larval NCB-LNvs at CT-15, biological rep3 |
| GSM682275 |
larval per0 NCB-LNvs at CT-15, biological rep1 |
| GSM682276 |
larval per0 NCB-LNvs at CT-15, biological rep2 |
| GSM682277 |
larval per0 NCB-LNvs at CT-15, biological rep3 |
| GSM682278 |
larval NCB-induced-LNvs at CT-15, biological rep1 |
| GSM682279 |
larval NCB--induced-LNvs at CT-15, biological rep2 |
| GSM682280 |
larval NCB--induced-LNvs at CT-15, biological rep3 |
| GSM682281 |
larval WT-induced-LNvs at CT-15, biological rep1 |
| GSM682282 |
larval WT-induced-LNvs at CT-15, biological rep2 |
| GSM682283 |
larval WT-induced-LNvs at CT-15, biological rep3 |
| GSM682284 |
larval Kir-LNvs at CT-3, biological rep1 |
| GSM682285 |
larval Kir-LNvs at CT-3, biological rep2 |
| GSM682286 |
larval Kir-LNvs at CT-3, biological rep3 |
| GSM682287 |
larval Kir-LNvs at CT-15, biological rep1 |
| GSM682288 |
larval Kir-LNvs at CT-15, biological rep2 |
| GSM682289 |
larval Kir-LNvs at CT-15, biological rep3 |
| GSM953019 |
larval cyc0Kir LNvs at CT-3, biological rep1 |
| GSM953020 |
larval cyc0Kir LNvs at CT-3, biological rep2 |
| GSM953021 |
larval cyc0Kir LNvs at CT-3, biological rep3 |
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| Relations |
| BioProject |
PRJNA138403 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE27565_RAW.tar |
43.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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