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| Status |
Public on Nov 24, 2024 |
| Title |
Gene expression dysregulation in whole blood of patients with C. difficile infection |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Background: Clostridioides difficile (C. difficile) is a global threat and has significant implications for individuals and health care systems. Little is known about host molecular mechanisms and transcriptional changes in peripheral immune cells. This is the first gene expression study in whole blood of patients with C. difficile infection. Methods: We took blood and stool samples from patients with toxigenic C. difficile infection (CDI), non-toxigenic C. difficile infection (GDH), inflammatory bowel disease (IBD), diarrhoea from other causes (DC) and healthy controls (HC). We performed transcriptome-wide RNA profiling on peripheral blood to identify diarrhoea common and CDI unique gene sets. Results: Diarrhoea groups upregulated innate immune responses with neutrophils at the epicentre. The common signature associated with diarrhoea was non-specific and shared by various other inflammatory conditions. CDI had a unique 45-gene set reflecting downregulation of humoral and T cell memory functions. Dysregulation of immunometabolic genes was also abundant and linked to immune cell fate during differentiation. Conclusions: Whole transcriptome analysis of white cells in blood from patients with toxigenic C. difficile infection showed that there is an impairment of adaptive immunity and immunometabolism.
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| Overall design |
278 whole blood samples were assayed, with some participants in the CDI group also measured ~2 weeks post-baseline. In total there are 78 samples from the CDI group at baseline, plus 27 at the 2-week time point; 45 samples from the diarrhoea from other causes (DC) group; 37 samples from the non-toxigenic C. difficile infection (GDH) group; 40 from the inflammatory bowel disease (IBD) group and 51 from the healthy control (HC) group.
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| Contributor(s) |
Tsakiroglou M, Evans A, Carracedo AD, Little M, Hornby R, Roberts P, Zhang E, Miyajima F, Pirmohamed M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Sep 05, 2024 |
| Last update date |
Nov 25, 2024 |
| Contact name |
Maria Tsakiroglou |
| Organization name |
University of Liverpool
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| Department |
Department of Pharmacology and Therapeutics
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| Street address |
Block A, Waterhouse Building, 1-5 Brownlow Street,
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| City |
Liverpool |
| ZIP/Postal code |
L69 3GL |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL23126 |
[Clariom_D_Human] Affymetrix Human Clariom D Assay [transcript (gene) version] |
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| Samples (278)
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| Relations |
| BioProject |
PRJNA1157210 |
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